antibody

Fli-1

MBS373012

5 + Control Slides

115 USD

monoclonal

mouse

nonconjugated

G146-22

Antibody

Fli-1

Fli-1

FLI-1; Friend leukemia integration 1 transcription factor; Proto-oncogene Fli-1; Transcription factor ERGB

Fli-1

FLI-1; FLI1

FLI1_HUMAN

Monoclonal

IgG1

G146-22

Mouse

Human

Fli-1 is a mouse monoclonal antibody derived from cell culture supernatant that is concentrated, dialyzed, filter sterilized and diluted in buffer pH 7.5, containing BSA and sodium azide as a preservative.

Store at 2 to 8 degree C. Stability: 3 years

Control: Angiosarcoma, Hemangiomas, PNET. Localization: Nuclear

Reactivity Note: Paraffin, Frozen

Fli-1 protein, a member of the ETS family of DNA binding transcription factors, is involved in cellular proliferation and tumorigenesis. Approximately 90% of Ewing's Sarcoma/Primitive Neuroectodermal Tumors (ES/PNET) have a specific translocation, t(11;22)(q24;q12), which results in fusion of EWS to Fli-1, and production of an EWS-Fli-1 fusion protein, which can be detected by this antibody. Among normal tissues only endothelial cells and small lymphocytes express Fli-1. Fli-1 has been found to be expressed in the great majority of vascular tumors including Angiosarcomas, Hemangioendotheliomas, Hemangiomas, and Kaposi's Sarcomas. It has been reported that the high sensitivity and specificity of Fli-1 is equal to or exceeds that of the established vascular markers, CD31, CD34, and Factor VIII. As the first nuclear marker of endothelium (rather than cytoplasmic or membranous), Fli-1 immunostaining also generally lacks cytoplasmic staining artifacts that are the result of endogenous peroxidases or biotin.

3821248

CAA76731.1

Y17293.1

Q01543

Focal Adhesion Pathway (83067); Focal Adhesion Pathway (478); MAPK Signaling Pathway (83048); MAPK Signaling Pathway (456); Salmonella Infection Pathway (375172); Salmonella Infection Pathway (375149)

This gene encodes a transcription factor containing an ETS DNA-binding domain. The gene can undergo a t(11;22)(q24;q12) translocation with the Ewing sarcoma gene on chromosome 22, which results in a fusion gene that is present in the majority of Ewing sarcoma cases. An acute lymphoblastic leukemia-associated t(4;11)(q21;q23) translocation involving this gene has also been identified. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2012]

Function: Sequence-specific transcriptional activator. Recognizes the DNA sequence 5'-C[CA]GGAAGT-3'. Subunit structure: Can form homodimers or heterodimers with ETV6/TEL1. Subcellular location: Nucleus. Involvement in disease: Defects in FLI1 are a cause of Ewing sarcoma (ES) [. MIM:612219]. A highly malignant, metastatic, primitive small round cell tumor of bone and soft tissue that affects children and adolescents. It belongs to the Ewing sarcoma family of tumors, a group of morphologically heterogeneous neoplasms that share the same cytogenetic features. They are considered neural tumors derived from cells of the neural crest. Ewing sarcoma represents the less differentiated form of the tumors. Note=A chromosomal aberration involving FLI1 is found in patients with Erwing sarcoma. Translocation t(11;22)(q24;q12) with EWSR1. Miscellaneous: Located on a fragment of chromosome 11 flanked on the centromeric side by the acute lymphoblastic leukemia-associated t(4;11)(q21;q23) translocation breakpoint and on the telomeric side by the Ewing- and neuroepithelioma-associated t(11;22) (q24;q12) breakpoint. Sequence similarities: Belongs to the ETS family.Contains 1 ETS DNA-binding domain.Contains 1 PNT (pointed) domain.

5 + Control Slides

115 USD

3 ml (Prediluted)

160

0.1 mL (Concentrate)

185

7 ml (Prediluted)

225

0.5 mL (Concentrate)

310