IDH1 R132H | MyBioSource MBS370356 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

IDH1 R132H

catalog :

MBS370356

quantity :

5 Control Slides

price :

130 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

[IHC132]

more info or order :

MyBioSource product webpage

image

image 1 :

IHC of IDHL 1 R132H on a FFPE Glioma Tissue

image 2 :

product information

catalog number :

MBS370356

products type :

Antibody

products full name :

IDH1 R132H

products short name :

[IDH1 R132H]

products gene name :

[IDH1 R132H]

clonality :

Monoclonal

isotype :

IgG1

clone :

[IHC132]

host :

Mouse

reactivity :

Human

form :

Prediluted

storage stability :

Storage: . Store at 2-8°C (Control Slides: Store at 20-25°C). Stability: . This product is stable up to the expiration date on the product label. Do not use after expiration date listed on package label. Temperature fluctuations should be avoided. Store appropriately when not in use, and avoid prolonged exposure to room temperature conditions.

tested application :

This antibody is intended for use in Immunohistochemical applications on formalin-fixed, paraffin-embedded tissues (FFPE), frozen tissue sections and cell preparations.

image1 heading :

Immunohistochemistry

image2 heading :

Protocol

other info1 :

Immunogen: Synthetic peptide corresponding to the human IDH1 surrounding residual 132. Localization: Cytoplasmic. Control: Glioma, Glioblastoma, Astrocytoma with IDH1 R132H Mutation

other info2 :

Presentation: ADHL 1 R132H is a mouse monoclonal antibody derived from cell culture supernatant that is concentrated, dialyzed, filter sterilized and diluted in buffer pH 7.5, containing BSA and sodium azide as a preservative. Specimen Preparation: Paraffin sections: . The antibody can be used on formalin-fixed paraffin-embedded (FFPE) tissue sections. Ensure tissue undergoes appropriate fixation for best results. Pre-treatment of tissues with heat-induced epitope retrieval (HIER) is recommended using ImmunoDNA Retriever with Citrate, ImmunoDNA Retriever with EDTA or ImmunoDNA Digestor. See reverse side for complete protocol. Tissue should remain hydrated via use of Immuno/DNA Washer solutions. Frozen sections and cell preparations: . The antibody can be used for labeling acetone-fixed frozen sections and acetone-fixed cell preparations. Staining Procedure: 1. Cut and mount 3-5 micron formalin-fixed paraffin-embedded tissues on positively charged slides such as Hydrophilic Plus Slides. 2. Air dry for 2 hours at 58°C. 3. Deparaffinize and rehydrate tissues. 4. Subject tissues to heat induced epitope retrieval (HIER) using a suitable retrieval solution such as ImmunoDNA Retriever with Citrate or EDTA. 5. Any of three heating methods may be used: . a. TintoRetriever Pressure Cooker or Equivalent . Place tissues/slides in a staining dish or coplin jar containing the ImmunoDNA Retriever with Citrate or EDTA, and place on trivet in the pressure cooker. Add 1-2 inches of distilled water to the pressure cooker and turn heat to high. Incubate for 15 minutes. Open and immediately transfer slides to room temperature. b. TintoRetriever PT Module or Water Bath Method . Place tissues/slides in a pre-warmed staining dish or coplin jar containing the ImmunoDNA Retriever with Citrate or EDTA at 95°-99°C. Incubate for 30-60 minutes. c. Conventional Steamer Method . Place tissues/slides in a pre-warmed staining dish or coplin jar containing the ImmunoDNA Retriever with Citrate or EDTA in a steamer, cover and steam for 30-60 minutes. 6. After heat treatment, transfer slides in ImmunoDNA Retriever with Citrate or EDTA to room temperature and let stand for 15-20 minutes. 7. For manual staining, perform antibody incubation at ambient temperature. For automated staining methods, perform antibody incubation according to instrument manufacturer’s instructions. 8. Wash slides with ImmunoDNA washer or DI water. 9. Continue IHC staining protocol. Wash slides between each step with ImmunoDNA washer solution. Mounting Protocols: For detailed instructions using biodegradable permanent mounting media such as XyGreen PermaMounter or organic solvent based resin such as PermaMounter refer to P10174 or P10097.

products description :

The IDH7 gene on chromosome band 2q33.3 encodes for isocitrate dehydrogenase 1 (IDH1), located in the cytoplasm and the peroxisomes. This enzyme catalyzes NADPH production via oxidative decarboxylation of isocitrate to alpha-ketoglutarate in the Krebs citric acid cycle. Studies have shown that IDH1 mutation is an early step in gliomagenesis and has been reported to occur in grades II and III astrocytomas, oligodendrogliomas (OG), oligoastrocytomas (OA), and secondary GBM. Mutations involving IDH1 occur in a high proportion of diffuse gliomas, with implications on diagnosis. About 90% involve exon 4 at codon 132, replacing amino acid arginine with histidine (R132H). Preliminary studies comparing Immunohistochemistry (IHC) with IDH1-R132H mutation-specific antibodies have shown concordance with DNA sequencing and no cross-reactivity with wild-type IDH1 or other mutant proteins.

size1 :

5 Control Slides

price1 :

130 USD

size2 :

3 ml (Prediluted)

price2 :

280

size3 :

0.1 mL (Concentrate)

price3 :

305

size4 :

7 mL (Prediluted)

price4 :

475

size5 :

0.5 mL (Concentrate)

price5 :

740

size6 :

15 mL (Prediluted)

price6 :

815

size7 :

1 mL (Concentrate)

price7 :

1090

more info or order :

MyBioSource product webpage