iNOS | MyBioSource MBS370310 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

iNOS

catalog :

MBS370310

quantity :

5 Control Slides

price :

130 USD

clonality :

monoclonal

host :

rabbit

conjugate :

nonconjugated

clone name :

[RBT-iNOS]

reactivity :

human, fruit fly

application :

immunohistochemistry, immunohistochemistry - paraffin section

more info or order :

MyBioSource product webpage

image

image 1 :

image 2 :

IHC of iNOS on an FFPE Liver Tissue

image 3 :

product information

catalog number :

MBS370310

products type :

Antibody

products full name :

iNOS

products short name :

[iNOS]

other names :

[inos; Inositol-3-phosphate synthase; CG11143 gene product from transcript CG11143-RA; Myo-inositol 1-phosphate synthase; IPS; MI-1-P synthase]

products gene name :

[iNOS]

other gene names :

[Inos; Inos; bs36h12.y1; CG11143; DmelCG11143; INOS; MIP synthase; IPS; MI-1-P synthase]

uniprot entry name :

INO1_DROME

clonality :

Monoclonal

isotype :

IgG

clone :

[RBT-iNOS]

host :

Rabbit

reactivity :

Human

sequence length :

565

form :

iNOS is a rabbit monoclonal antibody derived from cell culture supernatant that is concentrated, dialyzed, filter sterilized and diluted in buffer pH 7.5, containing BSA and sodium azide as a preservative.

storage stability :

Store at 2-8 °C. Do not use after expiration date listed on package label. Temperature fluctuations should be avoided. Store appropriately when not in use, and avoid prolonged exposure to room temperature condition.

tested application :

Immunohistochemistry (IHC) Formalin/Paraffin

image1 heading :

Dilution Information

image2 heading :

Immunohistochemistry (IHC)

image3 heading :

Recommended IHC Protocol

image4 heading :

Performance Characteristics

other info1 :

Intended Use: This antibody is intended for use in Immunohistochemical applications on formalin-fixed paraffin-embedded tissues (FFPE), frozen tissue sections and cell preparations. Interpretation of results should be performed by a qualified medical professional. Immunogen: Synthetic peptide corresponding to the N-terminus of the human iNOS protein. Localization: Cytoplasmic. Control: Testis, Adrenal, Lung, Prostate, Liver, Placenta, Spleen. Reactivity: Paraffin, Frozen

other info2 :

Specimen Preparation: Paraffin sections: The antibody can be used on formalin-fixed paraffin-embedded (FFPE) tissue sections. Ensure tissue undergoes appropriate fixation to ensure best results. Pre-treatment of tissues with heat-induced epitope retrieval (HIER) is recommended using ImmunoDNA Retriever with Citrate, ImmunoDNA Retriever with EDTA or ImmunoDNA Digestor. Tissue should remain hydrated using Immuno/DNA Washer solutions. Frozen sections and cell preparations: The antibody can be used for labeling acetone-fixed frozen sections and acetone-fixed cell preparations. Staining Procedure: 1. Cut and mount 3-5 micron formalin-fixed paraffin-embedded tissues on positive charged slides such as Hydrophilic Plus Slides. 2. Air dry for 2 hours at 58° C. 3. Deparaffinize, dehydrate and rehydrate tissues. 4. Subject tissues to heat epitope retrieval using a suitable retrieval solution such as ImmunoDNA Retriever with Citrate or EDTA. 5. Any of three heating methods may be used: . a. TintoRetriever Pressure Cooker or Equivalent Place tissues/slides in a staining dish or coplin jar containing the ImmunoDNA Retriever with Citrate or EDTA, and place in the pressure cooker. Add 1-2 inches of distilled water to the pressure cooker and turn heat to high. Incubate for 15 minutes. Open and immediately transfer slides to room temperature. b. TintoRetriever PT Module or Water Bath Method Place tissues/slides in a pre-warmed staining dish or coplin jar containing the ImmunoDNA Retriever with Citrate or EDTA at 95°-99° C. Incubate for 30-60 minutes. c. Conventional Steamer Method Place tissues/slides in a pre-warmed staining dish or coplin jar containing the ImmunoDNA Retriever with Citrate or EDTA in a Steamer, cover and steam for 30-60 minutes. 6. After heat treatment, transfer slides in ImmunoDNA Retriever with Citrate or EDTA to room temperature and let stand for 15-20 minutes. 7. For manual staining, perform antibody incubation at ambient temperature. For automated staining methods, perform antibody incubation according to instrument manufacturer’s instructions. 8. Wash slides with IHC wash buffer or DI water. 9. Continue IHC staining protocol. Precautions: 1. For professional users only. Ensure results are interpreted by a medical professional. 2.This product contains sodium azide (NaN3), a toxic chemical which may react with plumbing to form highly explosive build-ups of metal azides. Upon disposal, flush with large volumes of water to prevent sodium azide build-up. 3.Ensure proper handling procedures are used with reagent. Always wear proper laboratory equipment such as laboratory coat and gloves when handling reagents. 4.Unused solution should be disposed of according to local and federal regulations. 5.Do not ingest reagent. If reagent ingested, contact a poison control center immediately. Product Limitations: Due to inherent variability present in immunohistochemical procedures (including fixation time of tissues, dilution factor of antibody, retrieval method utilized and incubation time), optimal performance should be established through the use of positive and negative controls. Results should be interpreted by a medical professional.

products description :

Nitric oxide synthases (NOSs) are a family of enzymes catalyzing the production of nitric oxide (NO) from L-arginine. NO is an important cellular signaling molecule. It helps modulate vascular tone, insulin secretion, airway tone, and peristalsis, and is involved in angiogenesis and neural development.The inducible isoform, iNOS, is involved in immune response, binds calmodulin at physiologically relevant concentrations, and produces NO as an immune defense mechanism, as NO is a free radical with an unpaired electron. It is the proximate cause of septic shock and may function in autoimmune disease. The inducible isoform iNOS produces large amounts of NO as a defense mechanism. It is synthesized by many cell types in response to cytokines and is an important fac-tor in the response of the body to attack by parasites, bacterial infection, and tumor growth. It is also the cause of septic shock and may play a role in many diseases with an autoimmune etiology. Induction of the high-output iNOS usually occurs in an oxi-dative environment, and thus high levels of NO have the opportunity to react with superoxide leading to peroxynitrite formation and cell toxicity. These properties may define the roles of iNOS in host immunity, enabling its participation in anti-microbial and anti-tumor activities as part of the oxidative burst of macrophages.

ncbi gi num :

17137626

ncbi acc num :

NP_477405.1

ncbi gb acc num :

NM_058057.6

ncbi pathways :

Inositol Phosphate Metabolism Pathway (3024); Inositol Phosphate Metabolism Pathway (1372850); Inositol Phosphate Metabolism Pathway (362); Metabolic Pathways (132021); Metabolism Pathway (1372815); Synthesis Of IP2, IP, And Ins In The Cytosol Pathway (1372855)

size1 :

5 Control Slides

price1 :

130 USD

size2 :

0.1 mL (Concentrate)

price2 :

185

size3 :

3 ml (Prediluted)

price3 :

220

size4 :

0.5 mL (Concentrate)

price4 :

380

size5 :

7 mL (Prediluted)

price5 :

380

size6 :

1 mL (Concentrate)

price6 :

705

size7 :

15 mL (Prediluted)

price7 :

705

more info or order :

MyBioSource product webpage