antibody

CD68

MBS370295

5 Control Slides

130 USD

monoclonal

mouse

nonconjugated

[KP-1]

human, mouse, rat

immunohistochemistry, immunohistochemistry - paraffin section

Antibody

CD68

[CD68]

[CD68; Macrosialin; macrosialin; CD68 molecule; Gp110; CD_antigen: CD68]

[CD68]

[CD68; CD68; GP110; LAMP4; SCARD1]

CD68_HUMAN

Monoclonal

IgG1

[KP-1]

Mouse

Human, Hamster,Mouse, Non-Human Primate, Porcine, Rabbit, Rat

CD68 is a mouse monoclonal antibody derived from cell culture supernatant that is concentrated, dialyzed, filter sterilized and diluted in buffer pH 7.5, containing BSA and sodium azide as a preservative.

Store at 2-8°C. (Control Slides: Store at 20-25°C)

Immunohistochemistry (IHC) Formalin/Paraffin

This antibody is intended for use in Immunohistochemical applications on formalin-fixed paraffin-embedded tissues (FFPE), frozen tissue sections and cell preparations. Specimen Preparation: . Paraffin sections: The antibody can be used on formalin-fixed paraffin-embedded (FFPE) tissue sections. Ensure tissue undergoes appropriate fixation for best results. Pre-treatment of tissues with heat-induced epitope retrieval (HIER) is recommended using ImmunoDNA Retriever with Citrate , ImmunoDNA Retriever with EDTA or ImmunoDNA Digestor. See reverse side for complete protocol. Tissue should remain hydrated via use of Immuno/DNA Washer solutions. Frozen sections and cell preparations: The antibody can be used for labeling acetone-fixed frozen sections and acetone-fixed cell preparations. Staining Procedure: . 1. Cut and mount 3-5 micron formalin-fixed paraffin-embedded tissues on positively charged slides such as Hydrophilic Plus Slides. 2. Air dry for 2 hours at 58°C. 3. Deparaffinize, dehydrate and rehydrate tissues. 4. Subject tissues to heat induced epitope retrieval (HIER) using a suitable retrieval solution such as ImmunoDNA Retriever with Citrate or EDTA. 5. Any of three heating methods may be used:. a. TintoRetriever Pressure Cooker or Equivalent . Place tissues/slides in a staining dish or coplin jar containing the ImmunoDNA Retriever with Citrate or EDTA, and place on trivet in the pressure cooker. Add 1-2 inches of distilled water to the pressure cooker and turn heat to high. Incubate for 15 minutes. Open and immediately transfer slides to room temperature. b. TintoRetriever PT Module or Water Bath Method . Place tissues/slides in a pre-warmed staining dish or coplin jar containing the ImmunoDNA Retriever with Citrate or EDTA at 95°-99°C. Incubate for 30-60 minutes. c. Conventional Steamer Method . Place tissues/slides in a pre-warmed staining dish or coplin jar containing the ImmunoDNA Retriever with Citrate or EDTA in a steamer, cover and steam for 30-60 minutes. 6. After heat treatment, transfer slides in ImmunoDNA Retriever with Citrate or EDTA to room temperature and let stand for 15-20 minutes. 7. For manual staining, perform antibody incubation at ambient temperature. For automated staining methods, perform antibody incubation according to instrument manufacturer’s instructions. 8. Wash slides with ImmunoDNA washer or DI water. 9. Continue IHC staining protocol. Wash slides between each step with ImmunoDNA washer solution. Product Limitations: Due to inherent variability present in immunohistochemical procedures (including fixation time of tissues, dilution factor of antibody, retrieval method utilized and incubation time), optimal performance should be established through the use of positive and negative controls.

Dilution Information

Immunohistochemistry (IHC)

Recommended IHC Protocol

Performance Characteristics

Immunogen: Subcellular fraction of human alveolar macrophages. Localization: Cytoplasmic, Membranous. Control: Tonsil, Lymph Node. Intended Use: This antibody is intended for use in Immunohistochemical applications on formalinfixed paraffin-embedded tissues (FFPE), frozen tissue sections and cell preparations. Immunogen: Synthetic peptide corresponding to residues from the internal region of the human CD68 protein.

Precautions: 1. This product contains <0.1% sodium azide (NaN3) as a preservative. Ensure proper handling procedures are used with this reagent. 2. Always wear personal protective equipment such as laboratory coat, goggles and gloves when handling reagents. 3. Dispose of unused solution with copious amount of water. 4. Dispose of unused solution with copious amount of water. 5. Avoid contact with eyes. If contact occurs, flush with large quantities of water. 6. Follow safety precautions of the heating device used for epitope retrieval (TintoRetriever Pressure Cooker or similar). 7. For additional safety information refer to Safety Data Sheet for this product. 8. For complete recommendations for handling biological specimens, please refer to the CDC document, “Guidelines for Safe Work Practices in Human and Animal Medical Diagnostic Laboratories” (see References in this document). Mounting Protocols: For detailed instructions using biodegradable permanent mounting media such as XyGreen PermaMounter or organic solvent based resin such as PermaMounter, please inquire.

This antibody is intended for use in immunohistochemical applications on formalin fixed paraffin- embedded tissues (FFPE), frozen tissue sections and cell preparations. Interpretation of results should be performed by a qualified medical professional. The CD68 antigen is a heavily glycosylated trans-membrane protein of 87-115 kDa which is specifically expressed by tissue macrophages, Langerhans cells and, at low levels, by dendritic cells. CD68 could play a role in phagocytic activities of tissuemacrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. CD68 marks cells of monocyte/macrophage lineage. This antibody is capable of staining monocytes, Kupffer cells, osteoclasts, granulocytes and their precursors; Lymphomas are negative or show a few granules. This antibody may be useful for the identification of Myelomonocytic and Histiocytic Tumors. CD68 may help to distinguish Malignant Fibrous Histiocytoma from other Pleomorphic Sarcomas. However, since CD68 detects a formalin-resistant epitope that may be associated with lysosomal granules, other lysosome-rich cells may also produce positive results.

298665

AAB25811.1

Lysosome Pathway (99052); Lysosome Pathway (96865)

This gene encodes a 110-kD transmembrane glycoprotein that is highly expressed by human monocytes and tissue macrophages. It is a member of the lysosomal/endosomal-associated membrane glycoprotein (LAMP) family. The protein primarily localizes to lysosomes and endosomes with a smaller fraction circulating to the cell surface. It is a type I integral membrane protein with a heavily glycosylated extracellular domain and binds to tissue- and organ-specific lectins or selectins. The protein is also a member of the scavenger receptor family. Scavenger receptors typically function to clear cellular debris, promote phagocytosis, and mediate the recruitment and activation of macrophages. Alternative splicing results in multiple transcripts encoding different isoforms. [provided by RefSeq, Jul 2008]

CD68: Could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. Binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin-bearing substrates or other cells. Belongs to the LAMP family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Cell surface; Membrane protein, integral. Chromosomal Location of Human Ortholog: 17p13. Cellular Component: endosome membrane; integral to membrane; lysosomal membrane; membrane; plasma membrane

5 Control Slides

130 USD

3 ml (Prediluted)

180

0.1 mL (Concentrate)

190

7 mL (Prediluted)

195

0.5 mL (Concentrate)

275

15 mL (Prediluted)

310

1 mL (Concentrate)

445