NeuN | MyBioSource MBS370226 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

NeuN

catalog :

MBS370226

quantity :

5 Control Slides

price :

130 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

[A60]

more info or order :

MyBioSource product webpage

image

image 1 :

Inset: IHC of NeuN on a FFPE Brain Tissue

image 2 :

image 3 :

1. Cut and mount 3-5 micron formalin-fixed paraffin-embedded tissues on positive charged slides such as Hydrophilic Plus Slides. 2. Air dry for 2 hours at 58° C. 3. Deparaffinize, dehydrate and rehydrate tissues. 4. Subject tissues to heat epitope retrieval using a suitable retrieval solution such as ImmunoDNA Retriever with Citrate or EDTA. 5. Any of three heating methods may be used: . a. TintoRetriever Pressure Cooker or Equivalent . Place tissues/slides in a staining dish or coplin jar containing the ImmunoDNA Retriever with Citrate or EDTA, and place in the pressure cooker. Add 1-2 inches of distilled water to the pressure cooker and turn heat to high. Incubate for 15 minutes. Open and immediately transfer slides to room temperature. b. TintoRetriever PT Module or Water Bath Method . Place tissues/slides in a pre-warmed staining dish or coplin jar containing the ImmunoDNA Retriever with Citrate or EDTA at 95°-99° C. Incubate for 30-60 minutes. c. Conventional Steamer Method . Place tissues/slides in a pre-warmed staining dish or coplin jar containing the ImmunoDNA Retriever with Citrate or EDTA in a Steamer, cover and steam for 30-60 minutes. 6. After heat treatment, transfer slides in ImmunoDNA Retriever with Citrate or EDTA to room temperature and let stand for 15-20 minutes. 7. For manual staining, perform antibody incubation at ambient temperature. For automated staining methods, perform antibody incubation according to instrument manufacturer’s instructions. 8. Wash slides with IHC wash buffer or DI water. 9. Continue IHC staining protocol.

product information

catalog number :

MBS370226

products type :

Antibody

products full name :

NeuN

products short name :

[NeuN]

products gene name :

[NeuN]

clonality :

Monoclonal

isotype :

IgG1

clone :

[A60]

host :

Mouse

reactivity :

Human, Mouse, Rat, Chicken, Ferret, Pig

storage stability :

Store at 2-8 °C. Do not use after expiration date listed on package label. Temperature fluctuations should be avoided. Store appropriately when not in use, and avoid prolonged exposure to room temperature conditions.

image1 heading :

IHC

image2 heading :

Presentation

image3 heading :

Staining Procedure

image4 heading :

Performance Characteristics

other info1 :

Intended Use: This antibody is intended for use in Immunohistochemical applications on formalinfixed paraffin-embedded tissues (FFPE), frozen tissue sections and cell preparations. Interpretation of results should be performed by a qualified medical professional. Immunogen: Purified cell nuclei from mouse brain. Summary and Explanation: NeuN (Feminizing Locus on X-3, Fox-3, or Hexaribonucleotide Binding Protein-3) is a neuron-specific protein that is present in most Central Nervous System (CNS) and Peripheral Nervous System (PNS) neuronal cell types. NeuN protein distributions are restricted to neuronal nuclei, perikarya and some proximal neuronal processes in both fetal and adult brain. However, some neurons fail to be recognized by NeuN at all ages, such as INL retinal cells, Cajal-Retzius cells, Purkinje cells, inferior olivary and dentate nucleus neurons, and sympathetic ganglion cells. NeuN is widely used to label neurons since the vast majority of neurons are strongly positive. NeuN immunoreactivity becomes obvious as neurons mature, typically after they have downregulated expression of Doublecortin, a marker seen in the earliest stages of neuronal development.

other info2 :

Localization: Nuclear. Control: Brain. Presentation: Anti-NeuN is a mouse monoclonal antibody derived from cell culture supernatant that is concentrated, dialyzed, filter sterilized and diluted in buffer pH 7.5, containing BSA and sodium azide as a preservative. Precautions: 1. This product contains sodium azide (NaN3), a toxic chemical which may react with plumbing to form highly explosive build-ups of metal azides. Upon disposal, flush with large volumes of water to prevent sodium azide build-up. 2. Ensure proper handling procedures are used with reagent. Always wear proper laboratory equipment such as laboratory coat and gloves when handling reagents. 3. Unused solution should be disposed of according to local and federal regulations. 4. Do not ingest reagent. If reagent ingested, contact a poison control center immediately. 5. For complete recommendations for handling biological specimens please refer to the CDC document, “Guidelines for Safe Work Practices in Human and Animal Medical Diagnostic Laboratories” (5). Specimen Preparation: Paraffin sections: The antibody can be used on formalin-fixed paraffin-embedded (FFPE) tissue sections. Ensure tissue undergoes appropriate fixation to ensure best results. Pre-treatment of tissues with heat-induced epitope retrieval (HIER) is recommended using ImmunoDNA Retriever with Citrate, ImmunoDNA Retriever with EDTA or ImmunoDNA Digestor. Tissue should remain hydrated via use of Immuno/DNA Washer solutions. Frozen sections and cell preparations: The antibody can be used for labeling acetone-fixed frozen sections and acetone-fixed cell preparations.

size1 :

5 Control Slides

price1 :

130 USD

size2 :

3 ml (Prediluted)

price2 :

205

size3 :

0.1 mL (Concentrate)

price3 :

220

size4 :

7 mL (Prediluted)

price4 :

305

size5 :

0.5 mL (Concentrate)

price5 :

405

size6 :

15 mL (Prediluted)

price6 :

520

size7 :

1 mL (Concentrate)

price7 :

615

more info or order :

MyBioSource product webpage