other

SV40

MBS370184

5 Control Slides

180 USD

Antibody

SV40

[SV40]

[SV40]

Storage: Store at 20-25°C. Stability: This product is stable up to the expiration date on the product label. Do not use after expiration date listed on package label.

Immunohistochemistry Protocol

IHC

Presentation: Five slides of SV40 positive tissues, each mounted on Hydrophilic Plus Slides, provided in a plastic mailer.

Precautions: 1. Ensure proper handling procedures are used with reagent. 2. Always wear personal protective equipment such as laboratory coat, goggles, and gloves when handling reagents. 3. Dispose of unused material according to local and federal regulations. 4. Follow safety precautions of the heating device used for epitope retrieval (Tinto Retriever Pressure Cooker or similar). 5. For additional safety information refer to Safety Data Sheet for this product. 6. For complete recommendations for handling biological specimens please refer to the CDC document, "Guidelines for Safe Work Practices in Human and Animal Medical Diagnostic Laboratories" (see References in this document). Staining Procedure: 1. Subject tissues to heat induced epitope retrieval (HIER) using a suitable retrieval solution such as ImmunoDNA Retriever with Citrate, or ImmunoDNA Retriever EDTA. 2. Any ofthree heating methods may be used:. a. TintoRetriever Pressure Cooker or Equivalent Place tissues/slides in a staining dish or coplin jar containing the ImmunoDNA Retriever with Citrate or EDTA, and place on trivet in the pressure cooker. Add 1-2 inches of distilled water to the pressure cooker and turn heat to high. Incubate for 15 minutes. Open and immediately transfer slides to room temperature. b. TintoRetriever PT Module or Water Bath Method Place tissues/slides in a pre-warmed staining dish or coplin jar containing the ImmunoDNA Retriever with Citrate or EDTA at 95°-99° C. lncubate for 30-60 minutes. c. Conventional Steamer Method Place tissues/slides in a pre-warmed staining dish or coplin jar containing the ImmunoDNA Retriever with Citrate or EDTA in a steamer, cover and steam for 30-60 minutes. 3. After heat treatment, transfer slides in ImmunoDNA Retriever with Citrate or EDTA to room temperature and let stand for 15-20 minutes. 4. For manual staining, perform antibody incubation at ambient temperature. For automated staining methods, perform antibody incubation according to instrument manufacturer's instructions. 5. Wash slides with ImmunoDNA washer or DI water. 6. Continue IHC staining protocol. Wash slides between each step with ImmunoDNA washer solution. Mounting Protocols: For detailed instructions using biodegrad able permanent mounting media such as XyGreen PermaMounter or organic solvent based resin such as PermaMounter, please inquire. Product Limitations: Due to inherent variability present in immunohistochemical procedures (including fixation time of tissues, dilution factor of antibody, retrieval method utilized and incubation time), optimal performance should be established through the use of positive and negative controls.

SV40 is an abbreviation for Simian vacuolating virus 40 or Simian virus 40, a polyomavirus that is found in both monkeys and humans. Like other polyomaviruses, SV40 is a DNA virus that has the potential to cause tumors, but most often persists as a latent infection,

5 Control Slides

180 USD

0.1 mL (Concentrate)

220

3 ml (Prediluted)

275

0.5 mL (Concentrate)

410

7 mL (Prediluted)

465

15 mL (Prediluted)

705

1 mL (Concentrate)

715