Annexin A1 | MyBioSource MBS370183 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Annexin A1

catalog :

MBS370183

quantity :

5 Control Slides

price :

130 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

[BSB-95]

reactivity :

human

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS370183

products type :

Antibody

products full name :

Annexin A1

products short name :

[Annexin A1]

other names :

[annexin A1; Annexin A1; annexin A1; p35; annexin-1; calpactin-2; calpactin II; lipocortin I; chromobindin-9; annexin I (lipocortin I); phospholipase A2 inhibitory protein; annexin A1; Annexin I; Annexin-1; Calpactin II; Calpactin-2; Chromobindin-9; Lipocortin I; Phospholipase A2 inhibitory protein; p35]

other gene names :

[ANXA1; ANXA1; ANX1; LPC1; ANX1; LPC1]

uniprot entry name :

ANXA1_HUMAN

clonality :

Monoclonal

isotype :

IgG2b

clone :

[BSB-95]

host :

Mouse

reactivity :

Paraffin, Frozen

storage stability :

Store at 2-8 °C. Do not use after expiration date listed on package label. Temperature fluctuations should be avoided. Store appropriately when not in use, and avoid prolonged exposure to room temperature conditions.

other info1 :

Immunogen: Human Annexin A1. Location: Cytoplasmic, Membranous. Control: Liver, Tonsil, Spleen, Thymus, Lung, Colon, Hairy Cell Leukemia. Species Reactivity: Human, Dog

other info2 :

Staining Procedure: 1. Cut and mount 3-5 micron formalin-fixed paraffin-embedded tissues on positive charged slides such as Hydrophilic Plus Slides . 2. Air dry for 2 hours at 58° C. 3. Deparaffinize, dehydrate and rehydrate tissues. 4. Subject tissues to heat epitope retrieval using a suitable retrieval solution such as ImmunoDNA Retriever with Citrate . 5. Any of three heating methods may be used: . a. TintoRetriever r Pressure Cooker or Equivalent. Place tissues/slides in a staining dish or coplin jar containing the ImmunoDNA Retriever with Citrate or EDTA, and place in the pressure cooker. Add 1-2 inches of distilled water to the pressure cooker and turn heat to high. Incubate for 15 minutes. Open and immediately transfer slides to room temperature. b. TintoRetriever PT Module or Water Bath Method . Place tissues/slides in a pre-warmed staining dish or coplin jar containing the ImmunoDNA . Retriever with Citrate or EDTA at 95°-99° C. Incubate for 30-60 minutes. c. Conventional Steamer Method . Place tissues/slides in a pre-warmed staining dish or coplin jar containing the ImmunoDNA . Retriever with Citrate or EDTA in a Steamer, cover and steam for 30-60 minutes. 6. After heat treatment, transfer slides in ImmunoDNA Retriever with Citrate or EDTA to room temperature and let stand for 15-20 minutes. 7. For manual staining, perform antibody incubation at ambient temperature. For automated staining methods, perform antibody incubation according to instrument manufacturer’s instructions. 8. Wash slides with IHC wash buffer or DI water. 9. Continue IHC staining protocol. Precautions: 1. For professional users only. Ensure results are interpreted by a medical professional. 2. This product contains sodium azide (NaN3), a toxic chemical which may react with plumbing to form highly explosive build-ups of metal azides. Upon disposal, ?ush with large volumes of water to prevent sodium azide build-up. 3. Ensure proper handling procedures are used with reagent. Always wear proper laboratory equipment such as laboratory coat and gloves when handling reagents. 4. Unused solution should be disposed of according to local and federal regulations. 5. Do not ingest reagent. If reagent ingested, contact a poison control center immediately.

products description :

For In Vitro Diagnostic Use. This antibody is intended for use in Immunohistochemical applications on formalin-fixed paraffin-embedded tissues (FFPE), frozen tissue sections and cell preparations. Interpretation of results should be performed by a qualified medical professional. The protein Annexin A1 is encoded by the ANXA1 gene, which is upregulated in hairy cell leukemia. The NFkB signal transduction pathway is exploited by cancer - ous cells to proliferate and avoid apoptosis. Annexin A1 inhibits that pathway by binding to the p65 subunit, thus making Annexin A1 of particular interest for use as a potential anti-cancer drug. It may also contain tumor suppressive and protective characteristics, as has been evidenced by its ability to protect against DNA damage induced by heat in breast cancer cells. Annexin A1 is strongly expressed on the cell membrane and occasionally in the cytoplasm of tumor cells in 97% of samples from patients with hairy cell leukemia. By contrast, B-cell lymphomas other than hairy cell leukemia are ANXA1 negative. Thus, ANXA1 is a molecule specific to hairy cell leukemia that can be used to dif - ferentiate this disease from other B-cell lymphomas.

ncbi gi num :

4502101

ncbi acc num :

NP_000691.1

ncbi gb acc num :

NM_000700.1

uniprot acc num :

P04083

ncbi pathways :

Class A/1 (Rhodopsin-like Receptors) Pathway (106357); Formyl Peptide Receptors Bind Formyl Peptides And Many Other Ligands Pathway (161021); G Alpha (i) Signalling Events Pathway (119550); G Alpha (q) Signalling Events Pathway (106043); GPCR Downstream Signaling Pathway (119548); GPCR Ligand Binding Pathway (161020); Gastrin-CREB Signalling Pathway Via PKC And MAPK (645295); Integrated Breast Cancer Pathway (219801); Integrated Pancreatic Cancer Pathway (711360); Peptide Ligand-binding Receptors Pathway (106358)

ncbi summary :

Annexin I belongs to a family of Ca(2+)-dependent phospholipid binding proteins which have a molecular weight of approximately 35,000 to 40,000 and are preferentially located on the cytosolic face of the plasma membrane. Annexin I protein has an apparent relative molecular mass of 40 kDa, with phospholipase A2 inhibitory activity. Since phospholipase A2 is required for the biosynthesis of the potent mediators of inflammation, prostaglandins and leukotrienes, annexin I may have potential anti-inflammatory activity. [provided by RefSeq, Jul 2008]

uniprot summary :

Function: Calcium/phospholipid-binding protein which promotes membrane fusion and is involved in exocytosis. This protein regulates phospholipase A2 activity. It seems to bind from two to four calcium ions with high affinity. Subunit structure: Homodimer in placenta (20%); linked by transglutamylation. Interacts with DYSF . By similarity. Ref.7. Subcellular location: Nucleus . By similarity. Cytoplasm . By similarity. Cell projection cilium . By similarity. Basolateral cell membrane . By similarity. Note: Found in the cilium, nucleus and basolateral cell membrane of ciliated cells in the tracheal endothelium . By similarity. Found in the cytoplasm of type II pneumocytes and alveolar macrophages . By similarity. Domain: A pair of annexin repeats may form one binding site for calcium and phospholipid. Post-translational modification: Phosphorylated by protein kinase C, epidermal growth factor receptor/kinase and TRPM7. Phosphorylation results in loss of the inhibitory activity. Ref.5 Ref.8. Sequence similarities: Belongs to the annexin family.Contains 4 annexin repeats.

size1 :

5 Control Slides

price1 :

130 USD

size2 :

0.1 mL (Concentrate)

price2 :

170

size3 :

3 ml (Prediluted)

price3 :

180

size4 :

0.5 mL (Concentrate)

price4 :

235

size5 :

7 mL (Prediluted)

price5 :

265

size6 :

1 mL (Concentrate)

price6 :

375

size7 :

15 mL (Prediluted)

price7 :

425

more info or order :

MyBioSource product webpage