antibody

Caldesmon

MBS370151

3 ml

215 USD

monoclonal

mouse

nonconjugated

CALD-31

Antibody

Caldesmon

Caldesmon

caldesmon; Caldesmon; caldesmon; OTTHUMP00000207948; OTTHUMP00000207949; OTTHUMP00000207950; OTTHUMP00000207952; OTTHUMP00000207953; OTTHUMP00000207954; OTTHUMP00000207955; OTTHUMP00000214473; caldesmon 1

CALD1; CALD1; CDM; HCAD; LCAD; H-CAD; L-CAD; NAG22; MGC21352; CAD; CDM

CALD1_HUMAN

Monoclonal

IgG1/K

CALD-31

Mouse

Anti-Caldesmon (CALD-31) is a mouse monoclonal antibody derived from cell culture supernatant that is concentrated, dialyzed, filter sterilized and diluted in buffer pH 7.5, containing BSA and sodium azide as a preservative.

Store at 2 to 8 degree C in the dark.

Control: Appendix, Uterus, Leiomyoma. Localization: Cytoplasmic

Reactivity Note: Paraffin, Frozen

Caldesmon 1, also known as CALD1, is a human gene. Caldesmon is a calmodulin-binding protein. Like Calponin, Caldesmon tonically inhibits the ATPase activity of myosin in smooth muscle. This gene encodes a Calmodulin and actin-binding protein that play an essential role in the regulation of smooth muscle and nonmuscle contraction. Two closely-related variants of human Caldesmon have been identified. The h-Caldesmon variant (120 -150 kD) is predominantly expressed in smooth muscle, whereas I-Caldesmon (70 -80 kD) is found in non-muscle tissue and cells. Neither of the two variants has been detected in skeletal muscle. Anti-Caldesmon recognizes only the h-Caldesmon variant. Anti-Caldesmon antibody labels smooth muscle and tumors of smooth muscle, myofibroblastic, and myoepithelial differentiation. Anti-Caldesmon has also been used to differenti-ate Epithelioid Mesothelioma from Serous Papillary Carcinoma of the ovary.

180195

Q05682

93,231 Da

Muscle Contraction Pathway 106261!!Myometrial Relaxation And Contraction Pathways 198759!!Smooth Muscle Contraction Pathway 161025!!Vascular Smooth Muscle Contraction Pathway 96530!!Vascular Smooth Muscle Contraction Pathway 96866

This gene encodes a calmodulin- and actin-binding protein that plays an essential role in the regulation of smooth muscle and nonmuscle contraction. The conserved domain of this protein possesses the binding activities to Ca(2+)-calmodulin, actin, tropomyosin, myosin, and phospholipids. This protein is a potent inhibitor of the actin-tropomyosin activated myosin MgATPase, and serves as a mediating factor for Ca(2+)-dependent inhibition of smooth muscle contraction. Alternative splicing of this gene results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq]

Function: Actin- and myosin-binding protein implicated in the regulation of actomyosin interactions in smooth muscle and nonmuscle cells (could act as a bridge between myosin and actin filaments). Stimulates actin binding of tropomyosin which increases the stabilization of actin filament structure. In muscle tissues, inhibits the actomyosin ATPase by binding to F-actin. This inhibition is attenuated by calcium-calmodulin and is potentiated by tropomyosin. Interacts with actin, myosin, two molecules of tropomyosin and with calmodulin. Also play an essential role during cellular mitosis and receptor capping. Ref.6. Subcellular location: Cytoplasm cytoskeleton. By similarity. Cytoplasm myofibril. By similarity. Note: On thin filaments in smooth muscle and on stress fibers in fibroblasts (nonmuscle). Tissue specificity: High-molecular-weight caldesmon (isoform 1) is predominantly expressed in smooth muscles, whereas low-molecular-weight caldesmon (isoforms 2, 3, 4 and 5) are widely distributed in non-muscle tissues and cells. Not expressed in skeletal muscle or heart. Domain: The N-terminal part seems to be a myosin/calmodulin-binding domain, and the C-terminal a tropomyosin/actin/calmodulin-binding domain. These two domains are separated by a central helical region in the smooth-muscle form. Post-translational modification: In non-muscle cells, phosphorylation by CDK1 during mitosis causes caldesmon to dissociate from microfilaments. Phosphorylation reduces caldesmon binding to actin, myosin, and calmodulin as well as its inhibition of actomyosin ATPase activity. Phosphorylation also occurs in both quiescent and dividing smooth muscle cells with similar effects on the interaction with actin and calmodulin and on microfilaments reorganization. Sequence similarities: Belongs to the caldesmon family.

3 ml

215 USD