CD7 | MyBioSource MBS370119 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

CD7

catalog :

MBS370119

quantity :

5 Control Slides

price :

130 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

[LP15]

reactivity :

human

application :

immunohistochemistry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

more info or order :

MyBioSource product webpage

image

image 1 :

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product information

catalog number :

MBS370119

products type :

Antibody

products full name :

CD7

products short name :

[CD7]

other names :

[CD7 antigen protein; T-cell antigen CD7; T-cell antigen CD7; p41 protein; CD7 antigen (p41); T-cell leukemia antigen; T-cell surface antigen Leu-9; CD7 molecule; GP40; T-cell leukemia antigen; T-cell surface antigen Leu-9; TP41]

products gene name :

[CD7]

other gene names :

[CD7; CD7; GP40; TP41; Tp40; LEU-9]

uniprot entry name :

CD7_HUMAN

clonality :

Monoclonal

isotype :

IgG2b

clone :

[LP15]

host :

Mouse

reactivity :

Human

form :

CD7 is a mouse monoclonal antibody derived from cell culture supernatant that is concentrated, dialyzed, filter sterilized and diluted in buffer pH 7.5, containing BSA and sodium azide as a preservative.

storage stability :

Store at 2-8°C (Control Slides: Store at 20-25 degree C). Do not use after expiration date listed on package label. Temperature fluctuations should be avoided. Store appropriately when not in use, and avoid prolonged exposure to room temperature conditions.

tested application :

Immunohistochemistry (IHC) - Paraffin, Frozen.

app notes :

Intended Use: This antibody is intended for use in Immunohistochemical applications on formalinfixed paraffin-embedded tissues (FFPE), frozen tissue sections and cell preparations. Paraffin sections: The antibody can be used on formalin-fixed paraffin-embedded (FFPE) tissue sections. Ensure tissue undergoes appropriate fixation to ensure best results. Pre-treatment of tissues with heat-induced epitope retrieval (HIER) is recommended using ImmunoDNA Retriever with Citrate, ImmunoDNA Retriever with EDTA or ImmunoDNA Digestor. See reverse side for complete protocol. Tissue should remain hydrated via use of Immuno/DNA Washer solutions. Frozen sections and cell preparations: The antibody can be used for labeling acetone-fixed frozen sections and acetone-fixed cell preparations.

image1 heading :

Dilution Information

image2 heading :

Immunohistochemistry (IHC)

image3 heading :

IHC Protocol

other info1 :

Localization: Membranous. Control: Tonsil, Lymph Node. Immunogen: Full length of the human CD7 protein. Presentation: CD7 is a mouse monoclonal antibody derived from cell culture supernatant that is concentrated, dialyzed, filter sterilized and diluted in buffer pH 7.5, containing BSA and sodium azide as a preservative.

other info2 :

Precautions: 1. This product contains sodium azide (NaN3), a toxic chemical which may react with plumbing to form highly explosive build-ups of metal azides. Upon disposal, flush with large volumes of water to prevent sodium azide build-up. 2. Ensure proper handling procedures are used with reagent. Always wear proper laboratory equipment such as laboratory coat and gloves when handling reagents. 3. Unused solution should be disposed of according to local and federal regulations. 4. Do not ingest reagent. If reagent ingested, contact a poison control center immediately. 5. For complete recommendations for handling biological specimens please refer to the CDC document, “Guidelines for Safe Work Practices in Human and Animal Medical Diagnostic Laboratories”. Staining Procedure: 1. Cut and mount 3-5 micron formalin-fixed paraffin-embedded tissues on positive charged slides such as Hydrophilic Plus Slides. 2. Air dry for 2 hours at 58° C. 3. Deparaffinize, dehydrate and rehydrate tissues. 4. Subject tissues to heat epitope retrieval using a suitable retrieval solution such as ImmunoDNA Retriever with Citrate. 5. Any of three heating methods may be used: . a. TintoRetriever Pressure Cooker or Equivalent: . Place tissues/slides in a staining dish or coplin jar containing the ImmunoDNA Retriever with Citrate or EDTA, and place in the pressure cooker. Add 1-2 inches of distilled water to the pressure cooker and turn heat to high. Incubate for 15 minutes. Open and immediately transfer slides to room temperature. b. TintoRetriever PT Module or Water Bath Method: . Place tissues/slides in a pre-warmed staining dish or coplin jar containing the ImmunoDNA Retriever with Citrate or EDTA at 95°-99° C. Incubate for 30-60 minutes. c. Conventional Steamer Method: . Place tissues/slides in a pre-warmed staining dish or coplin jar containing the ImmunoDNA Retriever with Citrate or EDTA in a Steamer, cover and steam for 30-60 minutes. 6. After heat treatment, transfer slides in ImmunoDNA Retriever with Citrate or EDTA to room temperature and let stand for 15-20 minutes. 7. For manual staining, perform antibody incubation at ambient temperature. For automated staining methods, perform antibody incubation according to instrument manufacturer’s instructions. 8. Wash slides with IHC wash buffer or DI water. 9. Continue IHC staining protocol. Wash slides between each step with ImmunoDNA washer solution.

products description :

CD7 is a 40 kDa transmembrane, single-chain glycoprotein, which is a member of the immunoglobulin gene superfamily. It is expressed in the majority of immature and mature T-lymphocytes, and T-cell Leukemia. It is also found in natural killer cells, a small subpopulation of normal B-cells and in malignant B-cells. It plays an essential role in T-cell interactions and also in T-cell/B-cell interaction during early lymphoid development. CD7 is a consistently-expressed T-cell antigen in Lymphoblastic Lymphomas and Leukemias; therefore, it is a useful marker in the identification of such neoplastic proliferations. CD7 is expressed in the majority of mature peripheral T-cells, the majority of post-thymic T-cells, NK cells, some myeloid cells, T-cell Acute Lymphoblastic Leukemia/Lymphoma, Acute Myelogenous Leukemia and Chronic Myelogenous Leukemia. Interestingly, CD7 is conspicuously absent in adult T-cell Leukemia/Lymphoma and is not expressed in Sezary cells.

ncbi gi num :

180164

uniprot acc num :

P09564

ncbi mol weight :

25,409 Da

ncbi pathways :

Hematopoietic Cell Lineage Pathway (83078); Hematopoietic Cell Lineage Pathway (489)

ncbi summary :

This gene encodes a transmembrane protein which is a member of the immunoglobulin superfamily. This protein is found on thymocytes and mature T cells. It plays an essential role in T-cell interactions and also in T-cell/B-cell interaction during early lymphoid development. [provided by RefSeq]

uniprot summary :

Function: Not yet known. Subunit structure: Interacts with SECTM1. Ref.7. Subcellular location: Membrane; Single-pass type I membrane protein. Sequence similarities: Contains 1 Ig-like (immunoglobulin-like) domain.

size1 :

5 Control Slides

price1 :

130 USD

size2 :

0.1 mL (Concentrate)

price2 :

195

size3 :

3 ml (Prediluted)

price3 :

205

size4 :

7 mL (Prediluted)

price4 :

300

size5 :

0.5 mL (Concentrate)

price5 :

400

size6 :

15 mL (Prediluted)

price6 :

510

size7 :

1 mL (Concentrate)

price7 :

580

more info or order :

MyBioSource product webpage