Zap-70 | MyBioSource MBS370118 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Zap-70

catalog :

MBS370118

quantity :

5 + Control Slides

price :

115 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

2F3.2

reactivity :

human

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS370118

products type :

Antibody

products full name :

Zap-70

products short name :

Zap-70

other names :

tyrosine-protein kinase ZAP-70 isoform 1; Tyrosine-protein kinase ZAP-70; tyrosine-protein kinase ZAP-70; OTTHUMP00000203013; OTTHUMP00000216626; syk-related tyrosine kinase; 70 kDa zeta-associated protein; zeta-chain associated protein kinase, 70kD; zeta-chain (TCR) associated protein kinase 70kDa; 70 kDa zeta-associated protein; Syk-related tyrosine kinase

products gene name :

Zap-70

other gene names :

ZAP70; ZAP70; SRK; STD; TZK; ZAP-70; FLJ17670; FLJ17679; SRK

uniprot entry name :

ZAP70_HUMAN

clonality :

Monoclonal

isotype :

IgG2a

clone :

2F3.2

host :

Mouse

reactivity :

Human

form :

ZAP-70 is a mouse monoclonal antibody derived from cell culture supernatant that is concentrated, dialyzed, filter sterilized and diluted in buffer pH 7.5, containing BSA and sodium azide as a preservative.

storage stability :

Store at 2 to 8 degree C in the dark.

other info1 :

Control: Tonsil, Lymph Node, Chronic Lymphocytic Leukemia. Localization: Cytoplasmic

other info2 :

Reactivity Note: Paraffin, Frozen

products description :

ZAP-70 is an abbrevation for Zeta-chain-associated protein kinase 70 (70 is the molecular weight in kDa). The protein is a member of the protein-tyrosine kinase family. ZAP-70 is normally expressed in T-cells and natural killer cells and has a critical role in the initiation of T-cell signaling. ZAP-70 in B-cells is used as a prognostic marker in identifying different forms of Chronic Lymphocytic Leukemia (CLL). ZAP-70 protein is expressed in leukemic cells in approximately 25% of Chronic Lymphocytic Leukemia (CLL) cases as well. ZAP-70 expression is an excellent surrogate marker for the distinction between

ncbi gi num :

31455611

ncbi acc num :

NP_001070.2

ncbi gb acc num :

NM_001079.3

uniprot acc num :

P43403

ncbi mol weight :

69742 Da

ncbi pathways :

Adaptive Immunity Signaling Pathway (366160); B Cell Receptor Signaling Pathway (198909); Generation Of Second Messenger Molecules Pathway (106417); Immune System Pathway (106386); Inflammatory Response Pathway (198766); Natural Killer Cell Mediated Cytotoxicity Pathway (83079); Natural Killer Cell Mediated Cytotoxicity Pathway (490); Primary Immunodeficiency Pathway (83125); Primary Immunodeficiency Pathway (537); T Cell Receptor Signaling Pathway (198862)

ncbi summary :

This gene encodes an enzyme belonging to the protein tyrosine kinase family, and it plays a role in T-cell development and lymphocyte activation. This enzyme, which is phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation, functions in the initial step of TCR-mediated signal transduction in combination with the Src family kinases, Lck and Fyn. This enzyme is also essential for thymocyte development. Mutations in this gene cause selective T-cell defect, a severe combined immunodeficiency disease characterized by a selective absence of CD8-positive T-cells. Two transcript variants that encode different isoforms have been found for this gene. [provided by RefSeq]

uniprot summary :

Function: Plays a role in T-cell development and lymphocyte activation. Essential for TCR-mediated IL-2 production. Isoform 1 induces TCR-mediated signal transduction, isoform 2 does not. Ref.1 Ref.5. Catalytic activity: ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate. Subunit structure: Interacts with SLA2 when it is phosphorylated. Interacts with CD3Z and with phosphorylated NFAM1. Interacts with CBLB. By similarity. Interacts with CBL and SLA when it is phosphorylated. The association with SLA (or SLA2) and CBL probably leads to its destruction. Interacts with SHB. Interacts with DEF6. Interacts (via SH2 domains) with RHOH. By similarity. Interacts with FCRL3. Ref.1 Ref.8 Ref.9 Ref.10. Subcellular location: Cytoplasm. By similarity. Cell membrane. By similarity. Note: After antigen stimulation, isoform 1 concentrates at the immunological synapse and isoform 2 remains cytoplasmic. Co-localizes together with RHOH in the immunological synapse. RHOH is required for its proper localization to the cell membrane and cytoskeleton fractions in the thymocytes. Tissue specificity: Expressed in T- and natural killer cells. Ref.1. Domain: The SH2 domains bind to the phosphorylated tyrosine-based activation motif (TAM) of CD3Z and the non-canonical phosphorylated tyrosine-based activation motif (TAM) of RHOH. Post-translational modification: Phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation. Tyr-319 phosphorylation is essential for full activity. Ref.1 Ref.7 Ref.11 Ref.12 Ref.13 Ref.14 Ref.15 Ref.16. Involvement in disease: Defects in ZAP70 are the cause of selective T-cell defect (STD) [. MIM:176947]. STD is an autosomal recessive form of severe combined immunodeficiency characterized by a selective absence of CD8-type T-cells. Ref.5 Ref.19 Ref.20. Sequence similarities: Belongs to the protein kinase superfamily. Tyr protein kinase family. SYK/ZAP-70 subfamily.Contains 1 protein kinase domain.Contains 2 SH2 domains.

size1 :

5 + Control Slides

price1 :

115 USD

size2 :

3 ml (Prediluted)

price2 :

170

size3 :

0.1 mL (Concentrate)

price3 :

180

size4 :

7 ml (Prediluted)

price4 :

250

size5 :

0.5 mL (Concentrate)

price5 :

365

more info or order :

MyBioSource product webpage