antibody

Vimentin

MBS370116

3 ml

190 USD

monoclonal

mouse

nonconjugated

V9

Antibody

Vimentin

Vimentin

vimentin; Vimentin; vimentin; OTTHUMP00000019224; OTTHUMP00000019225; vimentin

VIM; VIM; FLJ36605

VIME_HUMAN

Monoclonal

IgG1/K

V9

Mouse

Vimentin is a mouse monoclonal antibody derived from cell culture supernatant that is concentrated, dialyzed, filter sterilized and diluted in buffer pH 7.5, containing BSA and sodium azide as a preservative.

Store at 2 to 8 degree C in the dark.

Control: Tonsil, Lymph Node. Localization: Cytoplasmic

Reactivity Note: Paraffin, Frozen

Vimentin is a member of the intermediate filament family of proteins. Intermediate filaments are an important structural feature of eukaryotic cells. Together with microtubules and actin microfilaments, they make up the cytoskeleton. Expression of vimentin, when used in conjunction with keratin, is helpful in distinguishing melanomas from Undifferentiated Carcinomas and Large-Cell Lymphomas. All Melanomas and Schwannomas react strongly with vimentin. This antibody recognizes a 57 kDa intermediate filament. It labels a variety of mesenchymal cells, including melanocytes, lymph cells, endothelial cells and fibroblasts. Non-reactivity of vimentin antibody is often considered more useful than its presence, since there are a few tumors that do not contain vimentin (e.g., Hepatoma and Seminoma).

62414289

NP_003371.2

NM_003380.2

P08670

53521 Da

Alpha6-Beta4 Integrin Signaling Pathway 198807!!Apoptosis Pathway 105648!!Apoptotic Cleavage Of Cellular Proteins Pathway 105678!!Apoptotic Execution Phase Pathway 105677!!Aurora B Signaling Pathway 138080!!Caspase Cascade In Apoptosis Pathway 137974!!Caspase-mediated Cleavage Of Cytoskeletal Proteins Pathway 105679!!Muscle Contraction Pathway 106261!!Striated Muscle Contraction Pathway 106262!!Striated Muscle Contraction Pathway 198903

This gene encodes a member of the intermediate filament family. Intermediate filamentents, along with microtubules and actin microfilaments, make up the cytoskeleton. The protein encoded by this gene is responsible for maintaining cell shape, integrity of the cytoplasm, and stabilizing cytoskeletal interactions. It is also involved in the immune response, and controls the transport of low-density lipoprotein (LDL)-derived cholesterol from a lysosome to the site of esterification. It functions as an organizer of a number of critical proteins involved in attachment, migration, and cell signaling. Mutations in this gene causes a dominant, pulverulent cataract.

Function: Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Subunit structure: Homopolymer. Interacts with HCV core protein. Interacts with LGSN and SYNM. Interacts (via rod region) with PLEC (via CH 1 domain). By similarity. Interacts with SLC6A4. Ref.22 Ref.35. Tissue specificity: Highly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone-independent mammary carcinoma cell lines. Ref.12 Ref.17. Post-translational modification: Filament disassembly during mitosis is promoted by phosphorylation at Ser-55 as well as by nestin. By similarity. One of the most prominent phosphoproteins in various cells of mesenchymal origin. Phosphorylation is enhanced during cell division, at which time vimentin filaments are significantly reorganized. Phosphorylation by PKN1 inhibits the formation of filaments. Sequence similarities: Belongs to the intermediate filament family. Sequence caution: The sequence BAB71275.1 differs from that shown. Reason: Intron retention.

3 ml

190 USD