antibody

Cytokeratin 20

MBS370051

3 ml

250 USD

monoclonal

mouse

nonconjugated

Ks20.8

Antibody

Cytokeratin 20

Cytokeratin 20

cytokeratin 20; Keratin, type I cytoskeletal 20; Cytokeratin-20; CK-20; Keratin-20; K20; Protein IT

KRT20

K1C20_HUMAN

Monoclonal

IgG2a/K

Ks20.8

Mouse

Cytokeratin 20 is a mouse monoclonal antibody derived from cell culture supernatant that is concentrated, dialyzed, filter sterilized and diluted in buffer pH 7.5, containing BSA and sodium azide as a preservative.

Store at 2 to 8 degree C in the dark.

Control: Colon Carcinoma, Colon Mucosa, Bladder. Localization: Cytoplasmic

Reactivity Note: Paraffin, Frozen

Cytokeratin 20 (CK20) is a 46 kDa intermediate filament protein whose expression is restricted primarily to gastric and intestinal epithelium, urothelium, and Merkel cells. Cytokeratin 20 is a Type I cytokeratin. It is a major cellular protein of mature enterocytes and goblet cells found in the gastric and intestinal mucosa. CK 20 is expressed in Adenocarcinomas of the colon, stomach, pancreas and biliary system. It is also expressed in Mucinous Ovarian Tumors, Transitional-cell Carcinomas of the urinary tract, and Merkel-cell Carcinomas. Cytokeratin 20 is useful in the differentiation of specific types of simple epithelial cells of the urinary tract and normal and malignantly-transformed epithelia. This antibody is essentially non-reactive in Squamous Cell Carcinomas and Adenocarcinomas of the Breast, Lung, and Endometrium, Non-mucinous Tumors of the Ovary, and Small-cell Carcinomas. This antibody is often used in conjunction with CK7 and other antibodies to distinguish Colon Carcinomas (CK20+) from Ovarian, Pulmonary, and Breast Carcinomas.

402645

P35900

48,487 Da

Focal Adhesion Pathway 83067!!Focal Adhesion Pathway 478!!MAPK Signaling Pathway 83048!!MAPK Signaling Pathway 456!!Salmonella Infection Pathway 375172!!Salmonella Infection Pathway 375149

Function: Plays a significant role in maintaining keratin filament organization in intestinal epithelia. When phosphorylated, plays a role in the secretion of mucin in the small intestine. By similarity. Ref.4 Ref.8. Subunit structure: Heterotetramer of two type I and two type II keratins. Associates with KRT8. Subcellular location: Cytoplasm Ref.6 Ref.7. Tissue specificity: Expressed predominantly in the intestinal epithelium. Expressed in luminal cells of colonic mucosa. Also expressed in the Merkel cells of keratinized oral mucosa; specifically at the tips of some rete ridges of the gingival mucosa, in the basal layer of the palatal mucosa and in the taste buds of lingual mucosa. Ref.6 Ref.7. Developmental stage: First detected at embryonic week 8 in individual 'converted' simple epithelial cells of the developing intestinal mucosa. In later fetal stages, synthesis extends over most goblet cells and a variable number of villus enterocytes. In the developing gastric and intestinal mucosa, expressed in all enterocytes and goblet cells as well as certain 'low-differentiated' columnar cells, whereas the neuroendocrine and Paneth cells are negative. Ref.1. Post-translational modification: Hyperphosphorylation at Ser-13 occurs during the early stages of apoptosis but becomes less prominent during the later stages. Phosphorylation at Ser-13 also increases in response to stress brought on by cell injury. By similarity.Proteolytically cleaved by caspases during apoptosis. Cleavage occurs at Asp-228. Ref.4. Miscellaneous: There are two types of cytoskeletal and microfibrillar keratin: I (acidic; 40-55 kDa) and II (neutral to basic; 56-70 kDa). Sequence similarities: Belongs to the intermediate filament family.

3 ml

250 USD