CD68 | MyBioSource MBS370038 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

CD68

catalog :

MBS370038

quantity :

5 Control Slides

price :

130 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

[CD68/G2]

more info or order :

MyBioSource product webpage

image

image 1 :

image 2 :

image 3 :

IHC of CD68 on an FFPE Tonsil Tissue

product information

catalog number :

MBS370038

products type :

Antibody

products full name :

CD68

products short name :

[CD68]

other names :

[CD68 antigen]

products gene name :

[CD68]

clonality :

Monoclonal

isotype :

IgG1

clone :

[CD68/G2]

host :

Mouse

reactivity :

Human, Mouse, Rabbit, Rat

form :

CD68 is a mouse monoclonal antibody derived from cell culture supernatant that is concentrated, dialyzed, filter sterilized and diluted in buffer pH 7.5, containing BSA and sodium azide as a preservative.

storage stability :

Store at 2-8 degree C. Do not use after expiration date listed on package label. Temperature fluctuations should be avoided. Store appropriately when not in use, and avoid prolonged exposure to room temperature conditions.

image1 heading :

Presentation

image2 heading :

IHC Protocol

image3 heading :

Immunohistochemistry (IHC)

other info1 :

Control: Tonsil, Lymph Node. Localization: Cytoplasmic, Membranous. Intended Use: For In Vitro Diagnostic Use. This antibody is intended for use in Immunohistochemical applications on formalinfixed paraffin-embedded tissues (FFPE), frozen tissue sections and cell preparations. Interpretation of results should be performed by a qualified medical professional. Immunogen: Synthetic peptide corresponding to residues from the internal region of the human CD68 protein. Precautions: 1. For professional users only. Ensure results are interpreted by a medical professional. 2. This product contains sodium azide (NaN3), a toxic chemical which may react with plumbing to form highly explosive build-ups of metal azides. Upon disposal, flush with large volumes of water to prevent sodium azide build-up. 3. Ensure proper handling procedures are used with reagent. Always wear proper laboratory equipment such as laboratory coat and gloves when handling reagents. 4. Unused solution should be disposed of according to local and federal regulations. 5. Do not ingest reagent. If reagent ingested, contact a poison control center immediately.

other info2 :

Reactivity Note: Paraffin, Frozen. Specimen Preparation: Paraffin sections: The antibody can be used on formalin-fixed paraffin-embedded (FFPE) tissue sections. Ensure tissue undergoes appropriate fixation to ensure best results. Pre-treatment of tissues with heat-induced epitope retrieval (HIER) is recommended using ImmunoDNA Retriever with Citrate, ImmunoDNA Retriever with EDTA or ImmunoDNA Digestor. See reverse side for complete protocol. Tissue should remain hydrated via use of Immuno/DNA Washer solutions. Frozen sections and cell preparations: The antibody can be used for labeling acetone-fixed frozen sections and acetone-fixed cell preparations. Staining Procedure: 1. Cut and mount 3-5 micron formalin-fixed paraffin-embedded tissues on positive charged slides such as Hydrophilic Plus Slides. 2. Air dry for 2 hours at 58° C. 3. Deparaffinize, dehydrate and rehydrate tissues. 4. Subject tissues to heat epitope retrieval using a suitable retrieval solution such as ImmunoDNA Retriever with Citrate or EDTA. 5. Any of three heating methods may be used:. a. TintoRetriever Pressure Cooker or Equivalent Place tissues/slides in a staining dish or coplin jar containing the ImmunoDNA Retriever with Citrate or EDTA, and place in the pressure cooker. Add 1-2 inches of distilled water to the pressure cooker and turn heat to high. Incubate for 15 minutes. Open and immediately transfer slides to room temperature. b. TintoRetriever PT Module or Water Bath MethodPlace tissues/slides in a pre-warmed staining dish or coplin jar containing the ImmunoDNA Retriever with Citrate or EDTA at 95°-99° . C. Incubate for 30-60 minutes. c. Conventional Steamer Method. Place tissues/slides in a pre-warmed staining dish or coplin jar containing the ImmunoDNA Retriever with Citrate or EDTA in . a Steamer, cover and steam for 30-60 minutes. 6. After heat treatment, transfer slides in ImmunoDNA Retriever with Citrate or EDTA to room temperature and let stand for 15-20 minutes. 7. Wash slides with IHC wash buffer or DI water. 8. Continue IHC staining protocol.

products description :

The CD68 antigen is a heavily glycosylated transmembrane protein of 87-115 kDa which is specifically expressed by tissue macrophages, Langerhans cells and, at low levels, by dendriticcells. CD68 could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracel-lular cell-cell and cell-pathogen interactions. CD68 marks cells of monocyte/macrophage lineage. This antibody is capable of staining monocytes, Kupffer cells, osteoclasts, granulocytes and their precursors; Lymphomas are negative or show a few granules. This antibody may be useful for the identification of Myelomonocytic and Histiocytic Tumors. CD68 may help to distinguish Malignant Fibrous Histiocytoma from other Pleomorphic Sarcomas. However, since CD68 detects a formalin-resistant epitope that may be associated with lysosomal granules, other lysosome-rich cells may also produce positive results.

ncbi gi num :

60653837

ncbi pathways :

Focal Adhesion Pathway (83067); Focal Adhesion Pathway (478); MAPK Signaling Pathway (83048); MAPK Signaling Pathway (456); Salmonella Infection Pathway (375172); Salmonella Infection Pathway (375149)

size1 :

5 Control Slides

price1 :

130 USD

size2 :

3 ml (Prediluted)

price2 :

170

size3 :

0.1 mL (Concentrate)

price3 :

180

size4 :

7 mL (Prediluted)

price4 :

185

size5 :

0.5 mL (Concentrate)

price5 :

255

size6 :

15 mL (Prediluted)

price6 :

285

size7 :

1 mL (Concentrate)

price7 :

410

more info or order :

MyBioSource product webpage