antibody

CA15-3

MBS370012

5 + Control Slides

115 USD

monoclonal

mouse

nonconjugated

DF3

Antibody

CA15-3

CA15-3

CA15-3

Monoclonal

IgG1

DF3

Mouse

Human

CA15-3 is a mouse monoclonal antibody derived from cell culture supernatant that is concentrated, dialyzed, filter sterilized and diluted in buffer pH 7.5, containing BSA and sodium azide as a preservative.

Store at 2 to 8 degree C. Stability: 3 years. For long-term storage of the concentrated antibody, it is recommended that aliquots of the antibody be frozen at -20 degree C in glycerol 50% (frost-freeze freezers are not recommended). Repeated freezing and thawing must be avoided. Dilute using an antibody diluent.

Control: Breast, Pancreas, Salivary Gland. Localization: Cytoplasmic, Membranous

Dilution Information: MBS370012.jpg. Immunohistochemistry (IHC) . Immunohistochemical: Recommended Immunohistochemical Protocol . Pretreatment: . 1. Cut and mount 3-4 micron formalin-fixed paraffin-embedded tissues on positive charged slides. 2. Air dry for 2 hours at 58 degree C. 3. Deparaffinize, dehydrate and rehydrate tissues. 4. Subject tissues to heat epitope retrieval using a suitable retrieval solution such as ImmunoDNA Retriever with Citrate or EDTA. 5. Any of three heating methods may be used: . a. Electric Pressure Cooker . Place tissues/slides in a staining dish or coplin jar containing the ImmunoDNA Retriever with Citrate or EDTA, and place in the presure cooker. Add 1-2 inches of distilled water to the pressure cooker and turn heat to high, and incubate for 15 minutes. Open and immediately transfer slides to room temperature. b. Water Bath Method . Place tissues/slides in a pre-warmed staining dish or coplin jar containing the ImmunoDNA Retriever with Citrate or EDTA in a water bath set at 95°-99° C. Incubate for 30-60 minutes. c. Conventional Steamer Method . Place tissues/slides in a pre-warmed staining dish or coplin jar containing the ImmunoDNA Retriever with Citrate or EDTA in a Steamer, cover and steam for 30-60 minutes. 6. After heat treatment, transfer slides in ImmunoDNA Retriever with Citrate or EDTA to room temperature and let stand for 15-20 minutes. 7. Wash slides with IHC wash buffer or DI water. 8. Continue IHC staining protocol.: MBS370012_IHC.jpg

This antibody has been used for evaluating the primary site of a metastatic carcinoma of unknown origin and distinguishing between benign and malignant lesions. It is believed that CA15-3 reacts primarily with the DF3-antigen, a 300 kDa mucin-like glycoprotein present on the apical border of secretory mammary epithelial cells. CA15-3 has been detected with immunohistochemistry in a wide spectrum of carcinomas, including Breast Carcinomas (ductal and lobular), Sarcomas (Synovial Sarcoma and Malignant Fibrous Histiocytomas), and Lung Carcinomas. CA15-3 can be used as a supplementary marker for epithelial differentiation. CA15-3 does not stain Melanomas or Ewing's Sarcomas. Approximately 30% of Hepatocellular Carcinomas are positive for CA15-3.

5 + Control Slides

115 USD

3 ml (Prediluted)

195

0.1 mL (Concentrate)

210

7 ml (Prediluted)

290

15 ml (Prediluted)

485