antibody

Actin, Muscle Specific

MBS370001

5 + Control Slides

115 USD

monoclonal

mouse

nonconjugated

HHF35

Antibody

Actin, Muscle Specific

Actin, Muscle Specific

Monoclonal

IgG1/K

HHF35

Mouse

Human, Dog, Cat, Mouse, Rat, Monkey, Rabbit, Chicken, Shrew

Actin, Muscle Specific is a mouse monoclonal antibody derived from cell culture supernatant that is concentrated, dialyzed, filter sterilized and diluted in buffer pH 7.5, containing BSA and sodium azide as a preservative.

Store at 2 to 8 degree C. Do not use after expiration date listed on the package label. Temperature fluctuations should be avoided. Store appropriately when not in use, and avoid prologed exposure to room temperature conditions.

Control: Skeletal Muscle, Appendix, Prostate. Localization: Cytoplasmic. Immunogen: SDS extract of human myocardium

Reactivity Note: Paraffin, Frozen. Recommended Immunohistochemical Protocol: Pretreatment . 1. Cut and mount 3-4 micron formalin-fixed paraffin-embedded tissues on positive charged slides. 2. Air dry for 2 hours at 58 degree C. 3. Deparaffinize, dehydrate and rehydrate tissues. 4. Subject tissues to heat epitope retrieval using a suitable retrieval solution such as ImmunoDNA Retriever . 5. Any of three heating methods may be used: . a. Electric Pressure Cooker . Place standoff rack at base of pressure cooker. Add 1 -2 inches of distilled water to the pressure cooker and turn heat to high, and incubate for 15 minutes. Open and immediately transfer slides to room temperature. b. Water Bath Method . Place.tissues/slides in a pre-warmed staining dish or coplin jar containing the ImmunoDNA Retriever with Citrate or EDTA in a water bath set at 95°-99° C.lncubate for 30-60 minutes. c. Conventional Steamer Method . Place tissues/slides in a pre-warmed staining dish or coplin jar containing the ImmunoDNA Retriever with Citrate or EDTA in a Steamer, cover and steam for 30-60 minutes. 6. After heat treatment, transfer slides in ImmunoDNA Retriever with Citrate or EDTA to room temperature and let stand for 15-20 minutes. 7. Wash slides with IHC wash buffer or DI water. 8. Continue IHC staining protocol.

Actin is a globular-structural, 345 kDa protein that polymerizes in a helical fashion to form an actin filament (or microfilament). Actin filaments provide mechanical support for the cell, determine the cell shape, enable cell movements (through lamellipodia, filopodia, or pseudopodia); and participate in certain cell junctions, in cytoplasmic streaming and in contraction of the cell during cytokinesis. In muscle cells they play an essential role, along with myosin, in muscle contraction. In the cytosol, actin is predominantly bound to ATP, but can also bind to ADP. This antibody recognizes actin of skeletal, cardiac, and smooth-muscle cells. It is not reactive with other mesenchymal cells except for myoepithelium. Muscle-Specific Actin recognizes alpha and gamma isotypes of all muscle groups. Non-muscle cells such as vascular endothelial cells and connective tissues are nonreactive. Neoplastic cells of non-muscle-derived tissue such as Carcinomas, Melanomas and Lymphomas are negative. This antibody is useful in the identification of rhabdoid cellular elements.

5 + Control Slides

115 USD

3 ml (Prediluted)

135

7 ml (Prediluted)

150

0.1 mL (Concentrate)

155

15 ml (Prediluted)

210