protein

Enterokinase (Bovine) (His tag)

MBS355467

100 Units

330 USD

Enzyme

Enterokinase (Bovine) (His tag)

[Enterokinase (Bovine)]

[Enteropeptidase, Enterokinase, Serine protease 7, ENTK]

[enteropeptidase; Enteropeptidase; enteropeptidase; enterokinase; serine protease 7; protease, serine, 7 (enterokinase); transmembrane protease, serine 15; Enterokinase; Serine protease 7; Transmembrane protease serine 15]

[TMPRSS15; TMPRSS15; ENTK; PRSS7; ENTK; PRSS7]

ENTK_HUMAN

Pichia pastoris

95% by HPLC

Lyophilized

Shipping: Blue ice. Upon receipt, store at -80°C for long term. Avoid repeated freeze and thaw cycles.

Instrumental Enzyme

Background/Introduction: Recombinant adenoviruses have tremendous potential in both research and therapeutic applications. There are numerous advantages they provide when introducing genetic material into host cells. The permissive host cell range is very wide. The virus has been used to infect many mammalian cell types (both replicative and non-replicative) for high expression of the recombinant protein. Recombinant adenoviruses are especially useful for gene transfer and protein expression in cell lines that have low transfection efficiency with liposome. After entering cells, the virus remains epichromosomal (i.e. does not integrate into the host chromosome so does not activate or inactivate host genes). Recently, recombinant adenoviruses have been used to deliver RNAi into cells. HEK 293 cells or their variants are used as host cells for viral amplification. Recombinant adenoviruses can be grown at high titer (1010 VP (viral particles)/mL, which can be concentrated up to 1013 VP/mL) and purified by ViraBind Adenoviral Purification Kit or traditional CsCl ultracentrifugation. A particular challenge in the delivery of a gene by a viral vector is the accurate measurement of virus titer. Traditionally, infectivity particles are measured in culture by a plaque-forming unit assay (PFU) that scores the number of viral plaques as a function of dilution. These methods are time-consuming (10 days), require a long infection period, and suffer from a high degree of inter-assay variability and are affected by virus-cell interactions. QuickTiter Adenovirus Titer ELISA Kit utilizes an antibody against adenovirus hexon proteins to quantitate infected cells. The hexon proteins are the largest and most abundant of the structural proteins in the adenovirus capsid, and they are distributed symmetrically to form capsid facets. QuickTiter Adenovirus Titer ELISA Kit provides a quick and complete system to functionally titer virus infectivity; it provides sufficient reagents for up to 192 tests in 96-well plates. In contrast to the 10-day infection of a classical plaque assay, the kit only requires a 2-day infection. Detection sensitivity is 104 ifu/mL (106-107 VP/mL), which is sufficient for most adenoviral samples. The kit recognizes all 41 serotypes of adenovirus by immunocytochemistry and can be used with any adenovirus system as long as the virus is able to amplify in HEK 293 cells.

223942069

NP_002763.2

NM_002772.2

P98073

This gene encodes an enzyme that converts the pancreatic proenzyme trypsinogen to trypsin, which activates other proenzymes including chymotrypsinogen and procarboxypeptidases. The precursor protein is cleaved into two chains that form a heterodimer linked by a disulfide bond. This protein is a member of the trypsin family of peptidases. Mutations in this gene cause enterokinase deficiency, a malabsorption disorder characterized by diarrhea and failure to thrive. [provided by RefSeq, Jul 2008]

Function: Responsible for initiating activation of pancreatic proteolytic proenzymes (trypsin, chymotrypsin and carboxypeptidase A). It catalyzes the conversion of trypsinogen to trypsin which in turn activates other proenzymes including chymotrypsinogen, procarboxypeptidases, and proelastases. Catalytic activity: Activation of trypsinogen by selective cleavage of 6-Lys- -Ile-7 bond. Subunit structure: Heterodimer of a catalytic (light) chain and a multidomain (heavy) chain linked by a disulfide bond. Subcellular location: Membrane; Single-pass type II membrane protein . Probable. Tissue specificity: Intestinal brush border. Post-translational modification: The chains are derived from a single precursor that is cleaved by a trypsin-like protease. Involvement in disease: Enterokinase deficiency (ENTKD) [MIM:226200]: Life-threatening intestinal malabsorption disorder characterized by diarrhea and failure to thrive.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.2. Sequence similarities: Belongs to the peptidase S1 family.Contains 2 CUB domains.Contains 2 LDL-receptor class A domains.Contains 1 MAM domain.Contains 1 peptidase S1 domain.Contains 1 SEA domain.Contains 1 SRCR domain. Sequence caution: The sequence CAB90389.1 differs from that shown. Reason: Erroneous gene model prediction.

100 Units

330 USD

500 Units

775