GM-CSF Rat ELISA Kit | MyBioSource MBS355457 product information

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product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

GM-CSF Rat ELISA Kit

catalog :

MBS355457

quantity :

96 Tests

price :

440 USD

product information

catalog number :

MBS355457

products type :

ELISA Kit

products full name :

GM-CSF Rat ELISA Kit

products short name :

[GM-CSF]

products name syn :

[Rat GM-CSF]

other names :

[GM-CSF; Granulocyte-macrophage colony-stimulating factor; granulocyte-macrophage colony-stimulating factor; CSF; molgramostin; sargramostim; colony stimulating factor 2 (granulocyte-macrophage); Colony-stimulating factor; CSF; Molgramostin; Sargramostim]

products gene name :

[GM-CSF]

other gene names :

[CSF2; CSF2; GMCSF; GMCSF; GM-CSF; CSF]

uniprot entry name :

CSF2_HUMAN

reactivity :

Rat

storage stability :

Store at 2-8 degree C for 4 months, or at -20 degree C for 8 months.

tested application :

Quantitative sELISA (EIA)

app notes :

For quantitative detection of GM-CSF in rat serum, plasama, body fluids, tissue lysates or cell culture supernate.

image1 heading :

Typical Testing Data/Standard Curve

other info1 :

Samples: Rat serum, plasama, body fluids, tissue lysates or cell culture supernate. Assay Type: Sandwich. Detection Range: 15.6 pg/ml-1000 pg/ml. Sensitivity: < 10 pg/ml

products description :

Principle of the Assay This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-GM-CSF polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-GM-CSF polyclonal antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the GM-CSF amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of GM-CSF can be calculated. Background/Introduction: Granulocyte-macrophage colony-stimulating factor (GM-CSF) is a protein secreted by macrophages, T cells, mast cells, NK cells, endothelial cells and fibroblasts. It is essential for the in vitro proliferation and differentiation of precursor cells into mature granulocytes and macrophages. GM-CSF stimulates stem cells to produce granulocytes (neutrophils, eosinophils, and basophils) and monocytes. GM-CSF is used as a medication to stimulate the production of white blood cells following chemotherapy. Recently, it has been evaluated in clinical trials for its potential as a vaccine adjuvant in HIV-infected patients. The preliminary results have been promising but GM-CSF is not presently FDA-approved for this purpose.

ncbi gi num :

183364

ncbi acc num :

AAA52578.1

uniprot acc num :

P04141

ncbi mol weight :

16,295 Da

ncbi pathways :

Amoebiasis Pathway (167324); Amoebiasis Pathway (167191); Calcineurin-regulated NFAT-dependent Transcription In Lymphocytes Pathway (137993); Calcium Signaling In The CD4+ TCR Pathway (137941); Cytokine Signaling In Immune System Pathway (366171); Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460); Cytokines And Inflammatory Response Pathway (198794); Fc Epsilon RI Signaling Pathway (83082); Fc Epsilon RI Signaling Pathway (493)

ncbi summary :

The protein encoded by this gene is a cytokine that controls the production, differentiation, and function of granulocytes and macrophages. The active form of the protein is found extracellularly as a homodimer. This gene has been localized to a cluster of related genes at chromosome region 5q31, which is known to be associated with interstitial deletions in the 5q- syndrome and acute myelogenous leukemia. Other genes in the cluster include those encoding interleukins 4, 5, and 13. [provided by RefSeq, Jul 2008]

uniprot summary :

Function: Cytokine that stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes. Subunit structure: Monomer. The signaling GM-CSF receptor complex is a dodecamer of two head-to-head hexamers of two alpha, two beta, and two ligand subunits. Ref.13. Subcellular location: Secreted. Polymorphism: Variant Ile-117 may be a risk factor for atopic asthma. Pharmaceutical use: Available under the names Leukine (Immunex) and Leucomax (Novartis). Used in myeloid reconstitution following bone marrow transplant, bone marrow transplant engraftment failure or delay, mobilization and following transplantation of autologous peripheral blood progenitor cells, and following induction chemotherapy in older adults with acute myelogenous leukemia. Sequence similarities: Belongs to the GM-CSF family.

size1 :

96 Tests

price1 :

440 USD