This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
MyBioSource
product type :
ELISA/assay
product name :
IL-12 P40 Human ELISA Kit
catalog :
MBS355454
quantity :
96 Tests
price :
440 USD
product information
catalog number :
MBS355454
products type :
ELISA Kit
products full name :
IL-12 P40 Human ELISA Kit
products short name :
[IL-12 P40]
products name syn :
[Human IL-12 P40]
products gene name :
[IL-12 P40]
reactivity :
Human
storage stability :
Store at 2-8 degree C for 4 months, or at -20 degree C for 8 months.
tested application :
Quantitative sELISA (EIA)
app notes :
For quantitative detection of IL-12(P40) in human serum, plasma, body fluids, tissue lysates or cell culture supernatants.
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: human serum, plasma, body fluids, tissue lysates or cell culture supernatants. Detection Range: 31.2 pg/ml -2000 pg/ml. Sensitivity: < 2 pg/ml
products description :
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- IL-12(P40) polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- IL-12(P40) polyclonal antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the IL-12(P40) amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of IL-12(P40) can be calculated. Background: Interleukin 12 (IL-12) is an interleukin that is naturally produced by dendritic cells, macrophages and human B-lymphoblastoid cells (NC-37) in response to antigenic stimulation. IL-12 is composed of a bundle of four alpha helices. It is a heterodimeric cytokine encoded by two separate genes, IL-12A (p35) and IL-12B (p40). IL-12 plays an important role in the activities of natural killer cells and T lymphocytes. It mediates enhancement of the cytotoxic activity of NK cells and CD8+ cytotoxic T lymphocytes. IL-12 also has anti-angiogenic activity, which means it can block the formation of new blood vessels. IL12 p40 exists as a monomer and as a homodimer. The IL12B gene contains 8 exons, and maps to 5q31-q33.
size1 :
96 Tests
price1 :
440 USD
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.

"MyBioSource's number 1 vision is to be the world's number 1 quality reagents provider."

Our goal is to provide researchers, scientists and customers alike with a one-stop-shop for all of their reagents needs, whether it is monoclonal antibody, polyclonal antibody, recombinant protein, peptide, etc...

"MyBioSource offers the best products at unbeatable prices."

Please spend a few minutes to browse our online catalogs and see the wide range of products available. We ship our products through our shipping/distribution facility in San Diego, California, USA.

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