ELISA/assay

BMP-4 Human ELISA Kit

MBS355443

96 Tests

465 USD

ELISA Kit

BMP-4 Human ELISA Kit

[BMP-4]

[Human BMP-4]

[bone morphogenetic protein 4 preproprotein; Bone morphogenetic protein 4; bone morphogenetic protein 4; BMP-4; BMP-2B; bone morphogenetic protein 2B; bone morphogenetic protein 4; Bone morphogenetic protein 2B]

[BMP-4]

[BMP4; BMP4; ZYME; BMP2B; OFC11; BMP2B1; MCOPS6; BMP2B; DVR4; BMP-4; BMP-2B]

BMP4_HUMAN

Human

Store at 2-8 degree C for 4 months, or at -20 degree C for 8 months.

Quantitative sELISA (EIA)

For quantitative detection of BMP-4 in human serum, plasma, body fluids, tissue lysates or cell culture supernates.

Typical Testing Data/Standard Curve (for reference only)

Samples: human serum, plasma, body fluids, tissue lysates or cell culture supernates. Detection Range: 62.5 pg/ml-4000 pg/ml. Sensitivity: < 4 pg/ml

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- BMP-4 polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- BMP-4 polyclonal antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the BMP-4 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of BMP-4 can be calculated. Background: Bone morphogenetic protein 4 (BMP-4) is a member of the transforming growth factor-beta superfamily of regulatory molecules. Bone morphogenetic proteins were originally identified by the ability of demineralized bone extract to induce endochondral osteogenesis in vivo in an extraskeletal site, and known for their ability to induce bone and cartilage development. In human embryonic development, BMP4 is a critical signaling molecule required for the early differentiation of the embryo and establishing of a dorsal-ventral axis. BMP4 is secreted from the dorsal portion of the notochord, and it acts in concert with sonic hedgehog (released from the ventral portion of the notochord) to establish a dorsal-ventral axis for the differentiation of later structures. Increase in expression of BMP4 has been associated with a variety of bone diseases, including the heritable disorder Fibrodysplasia Ossificans Progressiva.

157276597

NP_570912.2

NM_130851.2

P12644

46,555 Da

Adipogenesis Pathway (198832); Basal Cell Carcinoma Pathway (83113); Basal Cell Carcinoma Pathway (525); Cardiac Progenitor Differentiation Pathway (712094); Elastic Fibre Formation Pathway (730310); Extracellular Matrix Organization Pathway (576262); Glypican 3 Network Pathway (138084); Heart Development Pathway (198802); Hedgehog Signaling Pathway (83063); Hedgehog Signaling Pathway (474)

The protein encoded by this gene is a member of the bone morphogenetic protein family which is part of the transforming growth factor-beta superfamily. The superfamily includes large families of growth and differentiation factors. Bone morphogenetic proteins were originally identified by an ability of demineralized bone extract to induce endochondral osteogenesis in vivo in an extraskeletal site. This particular family member plays an important role in the onset of endochondral bone formation in humans, and a reduction in expression has been associated with a variety of bone diseases, including the heritable disorder Fibrodysplasia Ossificans Progressiva. Alternative splicing in the 5' untranslated region of this gene has been described and three variants are described, all encoding an identical protein. [provided by RefSeq, Jul 2008]

Function: Induces cartilage and bone formation. Also act in mesoderm induction, tooth development, limb formation and fracture repair. Acts in concert with PTHLH/PTHRP to stimulate ductal outgrowth during embryonic mammary development and to inhibit hair follicle induction . By similarity. Subunit structure: Homodimer; disulfide-linked . By similarity. Interacts with GREM2. Part of a complex consisting of TWSG1 and CHRD. Interacts with the serine proteases, HTRA1 and HTRA3; the interaction with either inhibits BMP4-mediated signaling. The HTRA protease activity is required for this inhibition . By similarity. Interacts with SOSTDC1. Ref.5. Subcellular location: Secreted extracellular space extracellular matrix. Tissue specificity: Expressed in the lung and lower levels seen in the kidney. Present also in normal and neoplastic prostate tissues, and prostate cancer cell lines. Involvement in disease: Microphthalmia, syndromic, 6 (MCOPS6) [MIM:607932]: A disease characterized by microphthalmia/anophthalmia associated with facial, genital, skeletal, neurologic and endocrine anomalies. Microphthalmia is a disorder of eye formation, ranging from small size of a single eye to complete bilateral absence of ocular tissues (anophthalmia). In many cases, microphthalmia/anophthalmia occurs in association with syndromes that include non-ocular abnormalities.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.7Non-syndromic orofacial cleft 11 (OFC11) [MIM:600625]: A birth defect consisting of cleft lips with or without cleft palate. Cleft lips are associated with cleft palate in two-third of cases. A cleft lip can occur on one or both sides and range in severity from a simple notch in the upper lip to a complete opening in the lip extending into the floor of the nostril and involving the upper gum.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.9. Sequence similarities: Belongs to the TGF-beta family.

96 Tests

465 USD