ELISA/assay

IL-4 Rat ELISA Kit

MBS355442

96 Tests

385 USD

ELISA Kit

IL-4 Rat ELISA Kit

[IL-4]

[Rat IL-4]

[interleukin-4; Interleukin-4; interleukin-4; IL-4; BSF-1; B-cell stimulatory factor 1; lymphocyte stimulatory factor 1; B-cell stimulatory factor 1; BSF-1; Lymphocyte stimulatory factor 1]

[IL-4]

[IL4; IL4; IL-4; BSF-1]

IL4_PIG

Rat

Store at 2-8 degree C for 4 months, or at -20 degree C for 8 months.

Quantitative sELISA (EIA)

For quantitative detection of IL-4 in rat serum, body fluids, tissue lysates or cell culture supernatants.

Typical Testing Data/Standard Curve (for reference only)

Samples: Human Serum, Plasma, Body Fluids, Tissue Lysates Or Cell Culture Supernatants. Assay Type: Sandwich. Detection Range: 15.6 pg/ml-1000 pg/ml. Sensitivity: < 1 pg/ml

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-IL-17 polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-IL-17 polyclonal antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the IL-17 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of IL-17 can be calculated.

55742622

NP_999288.1

NM_214123.1

Q04745

15,023 Da

Allograft Rejection Pathway (84530); Allograft Rejection Pathway (535); Asthma Pathway (84527); Asthma Pathway (532); Autoimmune Thyroid Disease Pathway (84528); Autoimmune Thyroid Disease Pathway (533); Cytokine-cytokine Receptor Interaction Pathway (84463); Cytokine-cytokine Receptor Interaction Pathway (460); Fc Epsilon RI Signaling Pathway (84494); Fc Epsilon RI Signaling Pathway (493)

Function: Participates in at least several B-cell activation processes as well as of other cell types. It is a costimulator of DNA-synthesis. It induces the expression of class II MHC molecules on resting B-cells. It enhances both secretion and cell surface expression of IgE and IgG1. It also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. Subcellular location: Secreted. Sequence similarities: Belongs to the IL-4/IL-13 family.

96 Tests

385 USD