ELISA/assay

IL-6 Mouse ELISA Kit

MBS355431

96 Tests

410 USD

ELISA Kit

IL-6 Mouse ELISA Kit

[IL-6]

[Mouse IL-6]

[interleukin-6; Interleukin-6; interleukin-6; IL-6; Interleukin 6 (interferon, beta 2); interleukin 6]

[IL-6]

[Il6; Il6; ILg6; Ifnb2; Il-6; IL-6]

IL6_RAT

Mouse

Store at 2-8 degree C for 4 months, or at -20 degree C for 8 months.

Quantitative sELISA (EIA)

For quantitative detection of IL-6 in mouse serum, body fluids, tissue lysates or cell culture supernates.

Typical Testing Data/Standard Curve (for reference only)

Samples: mouse serum, body fluids, tissue lysates or cell culture supernates. Detection Range: 15.6 pg/ml-1000 pg/ml. Sensitivity: < 1 pg/ml

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-IL-6 polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-IL-6 polyclonal antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the IL-6 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of IL-6 can be calculated. Background: Interleukin-6 (IL-6) is an immunoregulatory cytokine that activates a cell surface signaling assembly composed of IL6, IL6RA, and the shared signaling receptor gp130. It acts as both a pro-inflammatory and anti-inflammatory cytokine. IL-6 is one of the most important mediators of fever and of the acute phase response. It is capable of crossing the blood brain barrier and initiating synthesis of PGE2 in the hypothalamus, thereby changing the body's temperature setpoint. IL-6 is also essential for hybridoma growth and is found in many supplemental cloning media such as briclone. Inhibitors of IL-6 are used to treat postmenopausal osteoporosis. IL-6 is relevant to many diseases such as diabetes, atherosclerosis, depression, Alzheimer's Disease, systemic lupus erythematosus, prostate cancer, and rheumatoid arthritis.

7549769

NP_036721.1

NM_012589.2

P20607

24,357 Da

Adipogenesis Pathway (198479); African Trypanosomiasis Pathway (194377); African Trypanosomiasis Pathway (194323); Amoebiasis Pathway (167311); Amoebiasis Pathway (167191); Androgen Receptor Signaling Pathway (198529); Chagas Disease (American Trypanosomiasis) Pathway (147806); Chagas Disease (American Trypanosomiasis) Pathway (147795); Cytokine Signaling In Immune System Pathway (829152); Cytokine-cytokine Receptor Interaction Pathway (83443)

a cytokine involved in development and possibly in neurodegenerative processes [RGD, Feb 2006]

Function: Cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation Acts on B-cells, T-cells, hepatocytes, hematopoietic progenitor cells and cells of the CNS. Also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance . By similarity. Subcellular location: Secreted. Sequence similarities: Belongs to the IL-6 superfamily.

96 Tests

410 USD