ELISA/assay

CXCL9 / MIG Human ELISA Kit

MBS355396

96 Tests

570 USD

ELISA Kit

CXCL9 / MIG Human ELISA Kit

CXCL9 / MIG

Human CXCL9 / MIG

CXCL9 / MIG

Human

Store at 2-8 degree C for 4 months, or at -20 degree C for 8 months.

Quantitative sELISA (EIA)

For quantitative detection of CXCL9 in human serum, plasma, body fluids, tissue lysates or cell culture supernatants.

Assay Type: Sandwich. Detection Range: 31.2 pg/ml-2000 pg/ml. Sensitivity: < 2 pg/ml

Principle of the assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-CXCL9 polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-CXCL9 polyclonal antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the CXCL9 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of CXCL9 can be calculated. Background: Chemokine (C-X-C motif) ligand 9 (CXCL9) is a small cytokine belonging to the CXC chemokine family that is also known as Monokine induced by gamma interferon (MIG). CXCL9 is a T-cell chemoattractant, which is induced by IFN-gamma. It is closely related to two other CXC chemokines called CXCL10 and CXCL11, whose genes are located near the gene for CXCL9 on human chromosome 4. CXCL9, CXCL10 and CXCL11 all elicit their chemotactic functions by interacting with the chemokine receptor CXCR3.

96 Tests

570 USD