ELISA/assay

IL-3 Human ELISA Kit

MBS355387

96 Tests

410 USD

ELISA Kit

IL-3 Human ELISA Kit

[IL-3]

[Human IL-3]

[IL-3; Interleukin-3; interleukin-3; mast cell growth factor; mast-cell growth factor; P-cell stimulating factor; P-cell-stimulating factor; hematopoietic growth factor; multilineage-colony-stimulating factor; multipotential colony-stimulating factor; interleukin 3 (colony-stimulating factor, multiple); Hematopoietic growth factor; Mast cell growth factor; MCGF; Multipotential colony-stimulating factor; P-cell-stimulating factor]

[IL-3]

[IL3; IL3; IL-3; MCGF; MULTI-CSF; IL-3; MCGF]

IL3_HUMAN

Human

Store at 2-8 degree C for 4 months, or at -20 degree C for 8 months.

Quantitative sELISA (EIA)

For quantitative detection of IL-3 in human serum, body fluids, tissue lysate or cell culture supernate.

Typical Testing Data/Standard Curve (for reference only)

Assay Type: Sandwich. Detection Range: 15.6 pg/ml-1000 pg/ml. Sensitivity: < 1 pg/ml

. Principle of the assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-IL-3 polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-IL-3 polyclonal antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the IL-3 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of IL-3 can be calculated. Background: Interleukin 3 (IL-3) is a hematopoietic colony-stimulating factor that is capable of supporting the proliferation of a broad range of hematopoietic cell types. It is chemically synthesized by means of an automated peptide synthesizer and is shown to have the biological activities attributed to native IL-3. It is secreted by basophils and activated T cells to support growth and differentiation of T cells from the bone marrow in an immune response. It can improve the body's natural response to disease as part of the immune system. It acts by binding to the interleukin-3 receptor. IL3 infusion expands an early cell population that subsequently requires the action of a later-acting factor such as GMCSF to complete its development.

3002475

AAC08706.1

P08700

17,233 Da

Apoptosis Pathway (83060); Apoptosis Pathway (470); Asthma Pathway (83120); Asthma Pathway (532); Calcineurin-regulated NFAT-dependent Transcription In Lymphocytes Pathway (137993); Calcium Signaling In The CD4+ TCR Pathway (137941); Cytokine Signaling In Immune System Pathway (366171); Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460); Fc Epsilon RI Signaling Pathway (83082)

The protein encoded by this gene is a potent growth promoting cytokine. This cytokine is capable of supporting the proliferation of a broad range of hematopoietic cell types. It is involved in a variety of cell activities such as cell growth, differentiation and apoptosis. This cytokine has been shown to also possess neurotrophic activity, and it may be associated with neurologic disorders. [provided by RefSeq, Jul 2008]

Function: Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages.This CSF induces granulocytes, macrophages, mast cells, stem cells, erythroid cells, eosinophils and megakaryocytes. Subunit structure: Monomer. Subcellular location: Secreted. Tissue specificity: Activated T-cells, mast cells, natural killer cells. Sequence similarities: Belongs to the IL-3 family.

96 Tests

410 USD