E-Selectin Human ELISA Kit | MyBioSource MBS355367 product information

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product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

E-Selectin Human ELISA Kit

catalog :

MBS355367

quantity :

96 Tests

price :

385 USD

product information

catalog number :

MBS355367

products type :

ELISA Kit

products full name :

E-Selectin Human ELISA Kit

products short name :

[E-Selectin]

products name syn :

[Human E-Selectin]

other names :

[E-selectin; E-selectin; E-selectin; ELAM-1; endothelial adhesion molecule 1; CD62 antigen-like family member E; endothelial leukocyte adhesion molecule 1; leukocyte endothelial cell adhesion molecule 2; leukocyte-endothelial cell adhesion molecule 2; selectin E; CD62 antigen-like family member E; Endothelial leukocyte adhesion molecule 1; ELAM-1; Leukocyte-endothelial cell adhesion molecule 2]

other gene names :

[SELE; SELE; ELAM; ESEL; CD62E; ELAM1; LECAM2; ELAM1; ELAM-1; LECAM2]

uniprot entry name :

LYAM2_HUMAN

reactivity :

Human

storage stability :

Store at 2-8 degree C for 4 months, or at -20 degree C for 8 months.

tested application :

Quantitative sELISA (EIA)

app notes :

For quantitative detection of E-Selectin in serum, plasma, body fluids, tissue lysates or cell culture supernates.

image1 heading :

Typical Standard Curve/Testing Data

other info1 :

Samples: Serum, plasma, body fluids, tissue lysates or cell culture supernates. Assay Type: Quantitative Sandwich. Detection Range: 62.5 pg/ml-4000 pg/ml. Sensitivity: < 4 pg/ml

products description :

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- E-Selectin polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- E-Selectin polyclonal antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the E-Selectin amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of E-Selectin can be calculated. Background/Introduction: E-selectin, also known as CD62 antigen-like family member E (CD62E), endothelial-leukocyte adhesion molecule 1 (ELAM-1), or leukocyte-endothelial cell adhesion molecule 2 (LECAM2), is a cell adhesion molecule expressed only on endothelial cells activated by cytokines. The ELAM gene is present in single copy in the human genome and contains 14 exons spanning about 13 kb of DNA. E-selectin mediates the adhesion of tumor cells to endothelial cells, by binding to E-selectin ligands expressed by neutrophils, monocytes, eosinophils, memory-effector T-like lymphocytes, natural killer cells or cancer cells.

ncbi gi num :

187960042

ncbi acc num :

NP_000441.2

ncbi gb acc num :

NM_000450.2

uniprot acc num :

P16581

ncbi mol weight :

66,655 Da

ncbi pathways :

ATF-2 Transcription Factor Network Pathway (138006); African Trypanosomiasis Pathway (194384); African Trypanosomiasis Pathway (194323); Cell Adhesion Molecules (CAMs) Pathway (83069); Cell Adhesion Molecules (CAMs) Pathway (480); Cell Surface Interactions At The Vascular Wall Pathway (106062); Glucocorticoid Receptor Regulatory Network Pathway (138014); Hemostasis Pathway (106028); Malaria Pathway (152665); Malaria Pathway (152657)

ncbi summary :

The protein encoded by this gene is found in cytokine-stimulated endothelial cells and is thought to be responsible for the accumulation of blood leukocytes at sites of inflammation by mediating the adhesion of cells to the vascular lining. It exhibits structural features such as the presence of lectin- and EGF-like domains followed by short consensus repeat (SCR) domains that contain 6 conserved cysteine residues. These proteins are part of the selectin family of cell adhesion molecules. Adhesion molecules participate in the interaction between leukocytes and the endothelium and appear to be involved in the pathogenesis of atherosclerosis. [provided by RefSeq, Jul 2008]

uniprot summary :

Function: Cell-surface glycoprotein having a role in immunoadhesion. Mediates in the adhesion of blood neutrophils in cytokine-activated endothelium through interaction with PSGL1/SELPLG. May have a role in capillary morphogenesis. Ref.1. Subunit structure: Interacts with PSGL1/SELPLG and PODXL2 through the sialyl Lewis X epitope. PSGL1 sulfation appears not to be required for this interaction. Ref.9 Ref.12. Subcellular location: Membrane; Single-pass type I membrane protein. Polymorphism: A polymorphism in position 149 is associated with a higher risk of coronary artery disease (CAD). A significantly higher mutation frequency (Arg-149) is observed in patients with angiographically proven severe atherosclerosis compared with an unselected population (Ser-149). Sequence similarities: Belongs to the selectin/LECAM family.Contains 1 C-type lectin domain.Contains 1 EGF-like domain.Contains 6 Sushi (CCP/SCR) domains.

size1 :

96 Tests

price1 :

385 USD