ELISA/assay

CARDIOTROPHIN-1 / CT-1 Human ELISA Kit

MBS355361

96 Tests

495 USD

ELISA Kit

CARDIOTROPHIN-1 / CT-1 Human ELISA Kit

Cardiotrophin-1 / CT-1

Human Cardiotrophin-1 / CT-1

cardiotrophin-1 isoform 2; Cardiotrophin-1; cardiotrophin-1; cardiophin 1; cardiotrophin 1

CT-1

CTF1; CTF1; CT1; CT-1; CT-1

CTF1_HUMAN

Human

Store at 2-8 degree C for 2 months, or at -20 degree C for 4 months.

Quantitative sELISA (EIA)

For quantitative detection of CT-1 in human serum, body fluids, tissue lysates or cell culture supernatants.

Assay Type: Sandwich. Detection Range: 31.2 pg/ml-2000 pg/ml. Sensitivity: < 10 pg/ml

Principle of the assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-CT-1 polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-CT-1 polyclonal antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the CT-1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of CT-1 can be calculated. Background: Cardiotrophin-1 (CT-1) is a cytokine. CTF1 gene contains 3 exons, spans over 6 to 7 kb. It is a cardiac hypertrophic factor of 21.5 kDa and a protein member of the IL-6 cytokine family. It is highly expressed in the heart, skeletal muscle, prostate and ovary and to lower levels in lung, kidney, pancreas, thymus, testis and small intestine. CT-1 is associated with the pathophysiology of heart diseases, including hypertension, myocardial infarction, valvular heart disease, and congestive heart failure. This protein exerts its cellular effects by interacting with the glycoprotein 130 (gp130)/leukemia inhibitory factor receptor beta (LIFR) heterodimer.

216548056

NP_001136016.1

NM_001142544.1

Q16619

21,227 Da

Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460); Jak-STAT Signaling Pathway (83077); Jak-STAT Signaling Pathway (488); MicroRNAs In Cardiomyocyte Hypertrophy Pathway (198784)

The protein encoded by this gene is a secreted cytokine that induces cardiac myocyte hypertrophy in vitro. It has been shown to bind and activate the ILST/gp130 receoptor. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2008]

Function: Induces cardiac myocyte hypertrophy in vitro. Binds to and activates the ILST/gp130 receptor. Subcellular location: Secreted. Tissue specificity: Highly expressed in heart, skeletal muscle, prostate and ovary. Lower levels in lung, kidney, pancreas, thymus, testis and small intestine. Little or no expression in brain, placenta, liver, spleen, colon or peripheral blood leukocytes. Sequence similarities: Belongs to the IL-6 superfamily.

96 Tests

495 USD