catalog number :
MBS355345
products type :
ELISA Kit
products full name :
BDNF Rat ELISA Kit
products short name :
[BDNF]
products name syn :
[Rat BDNF]
other names :
[BDNF, partial]
products gene name :
[BDNF]
storage stability :
Store at 2-8 degree C for 4 months, or at -20 degree C for 8 months.
tested application :
Quantitative sELISA (EIA)
app notes :
For quantitative detection of BDNF in Rat serum, plasma, body fluids, tissue lysates or cell culture supernatants.
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Mouse Serum, Plasma, Body Fluids, Tissue Lysates Or Cell Culture Supernates. Assay Type: Sandwich. Detection Range: 46.9 pg/ml-3000 pg/ml. Sensitivity: < 10pg/ml
products description :
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-BAFF polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-BAFF polyclonal antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the BAFF amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of BAFF can be calculated.
ncbi acc num :
ACY54685.1