GDNF Mouse ELISA Kit | MyBioSource MBS355344 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

GDNF Mouse ELISA Kit

catalog :

MBS355344

quantity :

96 Tests

price :

465 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS355344

products type :

ELISA Kit

products full name :

GDNF Mouse ELISA Kit

products short name :

GDNF

products name syn :

Mouse GDNF

other names :

glial cell line-derived neurotrophic factor isoform 5; Glial cell line-derived neurotrophic factor; glial cell line-derived neurotrophic factor; ATF; astrocyte-derived trophic factor; glial cell derived neurotrophic factor; Astrocyte-derived trophic factor

products gene name :

GDNF

other gene names :

GDNF; GDNF; ATF1; ATF2; HSCR3; HFB1-GDNF; hGDNF; ATF

uniprot entry name :

GDNF_HUMAN

reactivity :

Mouse

storage stability :

Store at 2-8 degree C for 6 months.

tested application :

Quantitative sELISA (EIA)

app notes :

For quantitative detection of GDNF in mouse serum, plasma, urine, cell culture supernatant or tissue samples.

other info1 :

Assay Type: Sandwich. Detection Range: 2 pg/ml-80 pg/ml

products description :

Principle of the assay: This kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. The purified anti-GDNF antibody was pre-coated onto 96-well plates. And the HRP conjugated anti-GDNF antibody was used as detection antibodies. The standards, test samples and HRP conjugated detection antibody were added to the wells subsequently, mixed and incubated, then, unbound conjugates were washed away with wash buffer. TMB substrates (A & B) were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the GDNF amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of GDNF can be calculated. Background: Glial cell-derived neurotrophic factor, also known as GDNF, is a founding member of the GDNF family of ligands (GFL). In humans, it is encoded by the GDNF gene. This gene mapped to 5p13.3-p13.1. Mutations in this gene may be associated with Hirschsprung's disease. GDNF is a small protein that potently promotes the survival of many types of neurons. The most prominent feature of GDNF is its ability to support the survival of dopaminergic and motorneurons. It promotes the survival and differentiation of dopaminergic neurons in culture, and was able to prevent apoptosis of motor neurons induced by axotomy. It also regulates kidney development and spermatogenesis, and it affects alcohol consumption.

ncbi gi num :

493796159

ncbi acc num :

NP_001265027.1

ncbi gb acc num :

NM_001278098.1

uniprot acc num :

P39905

ncbi mol weight :

23,720 Da

ncbi pathways :

Axon Guidance Pathway (105688); Developmental Biology Pathway (477129); NCAM Signaling For Neurite Out-growth Pathway (105689); NCAM1 Interactions Pathway (105697); Signaling Events Regulated By Ret Tyrosine Kinase Pathway (137915); Spinal Cord Injury Pathway (739007)

ncbi summary :

This gene encodes a highly conserved neurotrophic factor. The recombinant form of this protein was shown to promote the survival and differentiation of dopaminergic neurons in culture, and was able to prevent apoptosis of motor neurons induced by axotomy. The encoded protein is processed to a mature secreted form that exists as a homodimer. The mature form of the protein is a ligand for the product of the RET (rearranged during transfection) protooncogene. Multiple transcript variants encoding different isoforms have been found for this gene. Mutations in this gene may be associated with Hirschsprung disease. [provided by RefSeq, Jun 2010]

uniprot summary :

Function: Neurotrophic factor that enhances survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake. Ref.1. Subunit structure: Homodimer; disulfide-linked. Ref.1 Ref.11 Ref.14. Subcellular location: Secreted Ref.10. Tissue specificity: In the brain, predominantly expressed in the striatum with highest levels in the caudate and lowest in the putamen. Isoform 2 is absent from most tissues except for low levels in intestine and kidney. Highest expression of isoform 3 is found in pancreatic islets. Isoform 5 is expressed at very low levels in putamen, nucleus accumbens, prefrontal cortex, amygdala, hypothalamus and intestine. Isoform 3 is up-regulated in the middle temporal gyrus of Alzheimer disease patients while isoform 2 shows no change. Ref.2 Ref.12. Induction: By cAMP, 12-O-tetradecanoylphorbol-13-acetate (TPA) and FGF2. Ref.10. Involvement in disease: Hirschsprung disease 3 (HSCR3) [MIM:613711]: A disorder of neural crest development characterized by absence of enteric ganglia along a variable length of the intestine. It is the most common cause of congenital intestinal obstruction. Early symptoms range from complete acute neonatal obstruction, characterized by vomiting, abdominal distention and failure to pass stool, to chronic constipation in the older child.Note: The disease may be caused by mutations affecting the gene represented in this entry. Ref.15 Ref.16 Ref.17 Ref.19Congenital central hypoventilation syndrome (CCHS) [MIM:209880]: Rare disorder characterized by abnormal control of respiration in the absence of neuromuscular or lung disease, or an identifiable brain stem lesion. A deficiency in autonomic control of respiration results in inadequate or negligible ventilatory and arousal responses to hypercapnia and hypoxemia.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.18. Sequence similarities: Belongs to the TGF-beta family. GDNF subfamily.

size1 :

96 Tests

price1 :

465 USD

more info or order :

MyBioSource product webpage