ELISA/assay

IL-8 Human ELISA Kit

MBS355339

96 Tests

415 USD

ELISA Kit

IL-8 Human ELISA Kit

IL-8

Human IL-8

interleukin-8; Interleukin-8; interleukin-8; IL-8; C-X-C motif chemokine 8; alveolar macrophage chemotactic factor I; alveolar macrophage-derived chemotactic factor-I; Alveolar macrophage chemotactic factor I; AMCF-I; C-X-C motif chemokine 8

IL-8

IL8; IL8; CXCL8; AMCF-I; CXCL8; IL-8; AMCF-I

IL8_PIG

Human

Store at 2-8 degree C for 4 months, or at -20 degree C for 8 months.

Quantitative sELISA (EIA)

For quantitative detection of IL-8 in human serum, plasma, body fluids, tissue lysates or cell culture supernatants.

Assay Type: Sandwich. Detection Range: 15.6 pg/ml-1000 pg/ml (body fluids, tissue lysates or cell culture supernates) 7.8 pg/ml-500 pg/ml (human serum, plasma). Sensitivity: < 1 pg/ml

Principle of the Assay This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-IL-8 polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-IL-8 polyclonal antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the IL-8 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of IL-8 can be calculated. Background/Introduction: Interleukin-8 (IL8) is a member of the CXC chemokine family. It is proinflammatory and primarily mediates the activation and migration of neutrophils into tissue from peripheral blood. It produced by macrophages, and also synthesized by endothelial cells. IL-8 is one of the major mediators of the inflammatory response. It is secreted by several cell types. It functions as a chemoattractant, and is also a potent angiogenic factor. Primary function of IL-8 is the induction of chemotaxis in its target cells, and it also recruits neutrophils to phagocytose the antigen, which triggers the antigen pattern toll-like receptors. IL-8 is often associated with inflammation. As an example, it has been cited as a proinflammatory mediator in gingivitis and psoriasis.

47523124

NP_999032.1

NM_213867.1

P26894

11,633 Da

Activation Of Genes By ATF4 Pathway (831482); Amoebiasis Pathway (167313); Amoebiasis Pathway (167191); Bladder Cancer Pathway (84522); Bladder Cancer Pathway (527); Chagas Disease (American Trypanosomiasis) Pathway (147807); Chagas Disease (American Trypanosomiasis) Pathway (147795); Chemokine Receptors Bind Chemokines Pathway (831622); Chemokine Signaling Pathway (98758); Chemokine Signaling Pathway (96864)

Function: IL-8 is a chemotactic factor that attracts neutrophils, basophils, and T-cells, but not monocytes. It is also involved in neutrophil activation. It is released from several cell types in response to an inflammatory stimulus. Subunit structure: Homodimer. Subcellular location: Secreted. Tissue specificity: Alveolar macrophages. Induction: By lipopolysaccharide (LPS). Post-translational modification: Citrullination at Arg-27 prevents proteolysis, and dampens tissue inflammation, it also enhances leukocytosis, possibly through impaired chemokine clearance from the blood circulation . By similarity. Sequence similarities: Belongs to the intercrine alpha (chemokine CxC) family.

96 Tests

415 USD