This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
MyBioSource
product type :
ELISA/assay
product name :
EGF Human ELISA Kit
catalog :
MBS355318
quantity :
96 Tests
price :
440 USD
product information
catalog number :
MBS355318
products type :
ELISA Kit
products full name :
EGF Human ELISA Kit
products short name :
[EGF]
products name syn :
[Human EGF]
other names :
[epidermal growth factor]
products gene name :
[EGF]
reactivity :
Human
storage stability :
Store at 2-8 degree C for 4 months, or at -20 degree C for 8 months.
tested application :
Quantitative sELISA (EIA)
app notes :
For quantitative detection of EGF in Human serum, body fluids, tissue lysates or cell culture supernates.
image1 heading :
Typical Testing Data/Standard Curve
other info1 :
Samples: Human serum, body fluids, tissue lysates or cell culture supernates. Assay Type: Sandwich. Detection Range: 4.7 pg/ml-300 pg/ml. Sensitivity: < 1 pg/ml
products description :
Principle of the Assay This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-EGF polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-EGF polyclonal antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the EGF amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of EGF can be calculated. Background/Introduction: Epidermal growth factor (EGF) is a growth factor that stimulates cell growth, proliferation, and differentiation by binding to its receptor EGFR. It has a profound effect on the differentiation of specific cells in vivo and is a potent mitogenic factor for a variety of cultured cells of both ectodermal and mesodermal origin. EGF is produced in abundance by the mouse submandibular gland, and it can be found in human platelets, macrophages, urine, saliva, milk, and plasma. It is a magnesiotropic hormone crucial for total body Mg(2+) balance. Futamura et al. (2002) found that chronic treatment of rats with haloperidol had no influence on EGF levels in the brain or serum.
ncbi gi num :
688072
ncbi acc num :
AAB32226.1
size1 :
96 Tests
price1 :
440 USD
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.

"MyBioSource's number 1 vision is to be the world's number 1 quality reagents provider."

Our goal is to provide researchers, scientists and customers alike with a one-stop-shop for all of their reagents needs, whether it is monoclonal antibody, polyclonal antibody, recombinant protein, peptide, etc...

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Please spend a few minutes to browse our online catalogs and see the wide range of products available. We ship our products through our shipping/distribution facility in San Diego, California, USA.

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