product summary
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company name :
MyBioSource
product type :
ELISA/assay
product name :
MMP-1 Human ELISA Kit
catalog :
MBS355314
quantity :
96 Tests
price :
465 USD
more info or order :
product information
catalog number :
MBS355314
products type :
ELISA Kit
products full name :
MMP-1 Human ELISA Kit
products short name :
MMP-1
products name syn :
Human MMP-1
other names :
Mmp-1
products gene name :
MMP-1
reactivity :
Human
storage stability :
Store at 2-8 degree C for 4 months, or at -20 degree C for 8 months.
tested application :
Quantitative sELISA (EIA)
app notes :
For quantitative detection of MMP-1 in human serum, plasma, body fluids, tissue lysates or cell culture supernatants.
other info1 :
Assay Type: Sandwich. Detection Range: 156 pg/ml-10,000 pg/ml. Sensitivity: < 10pg/ml
products description :
Priciple of the assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-MMP-1 polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-MMP-1 polyclonal antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the MMP-1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of MMP-1 can be calculated. Background: Matrix metalloproteinase-1 (MMP-1), also known as interstitial collagenase and fibroblast collagenase[1], is a member of the matrix metalloproteinases family. Matrix metalloproteinases are zinc-dependent proteases that degrade extracellular matrix proteins. MMP-1 is structurally formed by different proteic building blocks. The Catalytic Domain of MMP-1 is composed of five highly twisted beta-strands (sI-sV), three alpha-helix (hA-hC) and a total of eight loops, enclosing a total of five metal ions, three Ca2+ and two Zn2+, one of which with catalytic role.[2] MMP1 in the stromal-tumor microenvironment can alter the behavior of cancer cells through PAR1 to promote cell migration and invasion.[3] The biologic implications of MMP1 acting as a molecular ratchet tethered to the cell surface suggested novel mechanisms for its role in tissue remodeling and cell-matrix interaction.
ncbi gi num :
384131858
ncbi acc num :
YP_005514470.1
ncbi gb acc num :
NC_017162.1
size1 :
96 Tests
price1 :
465 USD
more info or order :
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.
"MyBioSource's number 1 vision is to be the world's number 1 quality reagents provider."
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