ELISA/assay

IL-6 Human ELISA Kit

MBS355306

96 Tests

410 USD

ELISA Kit

IL-6 Human ELISA Kit

[IL-6]

[Human IL-6]

[interleukin-6; Interleukin-6; interleukin-6; IL-6; Interleukin 6 (interferon, beta 2); interleukin 6]

[IL-6]

[Il6; Il6; ILg6; Ifnb2; Il-6; IL-6]

IL6_RAT

Human

Store at 2-8 degree C for 4 months, or at -20 degree C for 8 months.

Quantitative sELISA (EIA)

For quantitative detection of IL-6 in human serum, plasma, body fluids, tissue lysates or cell culture supernates.

Typical Testing Data/Standard Curve (for reference only)

Samples: Rat Serum, Body Fluids, Tissue Lysates Or Cell Culture Supernatants. Assay Type: Sandwich. Detection Range: 15.6 pg/ml-1000 pg/ml. Sensitivity: < 1pg/ml

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-IL-4 polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-IL-4 polyclonal antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the IL-4 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of IL-4 can be calculated!!Background/Introduction: Interleukin-4(IL-4) is an 18-kD glycoprotein, it has 4 exons and is approximately 10 kb. It has a compact, globular fold, stabilised by 3 disulphide bonds. One half of the structure is dominated by a 4 alpha-helix bundle with a left-handed twist. It has many biological roles, including the stimulation of activated B-cell and T-cell proliferation, and the differentiation B cells into Plasma Cells, and it is a key regulator in humoral and adaptive immunity. IL-4 also has been shown to drive mitogenesis, dedifferentiation, and metastasis in rhabdomyosarcoma.

7549769

NP_036721.1

NM_012589.2

P20607

24,357 Da

Adipogenesis Pathway (198479); African Trypanosomiasis Pathway (194377); African Trypanosomiasis Pathway (194323); Amoebiasis Pathway (167311); Amoebiasis Pathway (167191); Androgen Receptor Signaling Pathway (198529); Chagas Disease (American Trypanosomiasis) Pathway (147806); Chagas Disease (American Trypanosomiasis) Pathway (147795); Cytokine Signaling In Immune System Pathway (829152); Cytokine-cytokine Receptor Interaction Pathway (83443)

a cytokine involved in development and possibly in neurodegenerative processes [RGD, Feb 2006]

Function: Cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation Acts on B-cells, T-cells, hepatocytes, hematopoietic progenitor cells and cells of the CNS. Also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance . By similarity. Subcellular location: Secreted. Sequence similarities: Belongs to the IL-6 superfamily.

96 Tests

410 USD