ELISA/assay

IL-15 Human ELISA Kit

MBS355305

96 Tests

440 USD

ELISA Kit

IL-15 Human ELISA Kit

[IL-15]

[Human IL-15]

[interleukin-15; Interleukin-15; interleukin-15; IL-15; interleukin 15]

[IL-15]

[Il15; Il15; IL-15]

IL15_RAT

Human

Store at 2-8 degree C for 4 months, or at -20 degree C for 8 months.

Quantitative sELISA (EIA)

For quantitative detection of IL-15 in human serum, plasma, body fluids, tissue lysates or cell culture supernatants.

Typical Testing Data/Standard Curve (for reference only)

Samples: Rat Serum, Body Fluids, Tissue Lysates Or Cell Culture Supernatants. Assay Type: Sandwich. Detection Range: 31.2 pg/ml-2000 pg/ml

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-IL-10 polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-IL-10 polyclonal antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the IL-10 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of IL-10 can be calculated!!Background/Introduction: Interleukin-10 (IL-10 or IL10) also known as human cytokine synthesis inhibitory factor (CSIF), is an anti-inflammatory cytokine. In humans, IL-10 is encoded by the IL10 gene, which is located on chromosome 1 and comprises 5 exons. The IL-10 protein is a homodimer, each of its subunits is 178-amino-acid long. It is primarily produced by monocytes. IL-10 is a cytokine with pleiotropic effects in immunoregulation and inflammation. It could downregulates the expression of Th1 cytokines, MHC class II antigens, and co-stimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production.

6981092

NP_037261.1

NM_013129.2

P97604

18,804 Da

Cytokine-cytokine Receptor Interaction Pathway (83443); Cytokine-cytokine Receptor Interaction Pathway (460); Cytokines And Inflammatory Response (BioCarta) Pathway (198521); HTLV-I Infection Pathway (373894); HTLV-I Infection Pathway (373889); Herpes Simplex Infection Pathway (377869); Herpes Simplex Infection Pathway (377865); Intestinal Immune Network For IgA Production Pathway (128752); Intestinal Immune Network For IgA Production Pathway (128670); Jak-STAT Signaling Pathway (83469)

human homolog is a cytokine involved in the regulation of T and natural killer cell activation and proliferation [RGD, Feb 2006]

Function: Cytokine that stimulates the proliferation of T-lymphocytes. Stimulation by IL-15 requires interaction of IL-15 with components of IL-2R, including IL-2R beta and probably IL-2R gamma but not IL-2R alpha . By similarity. Subcellular location: Secreted. Sequence similarities: Belongs to the IL-15/IL-21 family.

96 Tests

440 USD