This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
MyBioSource
product type :
ELISA/assay
product name :
IL-10 Human ELISA Kit
catalog :
MBS355300
quantity :
96 Tests
price :
385 USD
product information
catalog number :
MBS355300
products type :
ELISA Kit
products full name :
IL-10 Human ELISA Kit
products short name :
[IL-10]
products name syn :
[Human IL-10]
other names :
[IL-10; IL-10]
products gene name :
[IL-10]
other gene names :
[SPPV_03]
reactivity :
Human
storage stability :
Store at 2-8 degree C for 4 months, or at -20 degree C for 8 months.
tested application :
Quantitative sELISA (EIA)
app notes :
For quantitative detection of IL-10in human serum, body fluids, tissue lysates or cell culture supernatants.
image1 heading :
Typical Testing Data/Standard Curve
other info1 :
Assay Type: Sandwich. Detection Range: 7.8 pg/ml-500 pg/ml (body fluids, tissue lysates or cell culture supernates) 3.4pg/ml-250pg/ml (human serum, plasma). Sensitivity: < 0.5 pg/ml
products description :
Principle of the Assay This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-IL-10polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-IL-10polyclonal antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the IL-10amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of IL-10can be calculated. Background/Introduction: Interleukin-10 (IL-10 or IL10) also known as human cytokine synthesis inhibitory factor (CSIF), is an anti-inflammatory cytokine. In humans, IL-10 is encoded by the IL10 gene, which is located on chromosome 1 and comprises 5 exons. The IL-10 protein is a homodimer, each of its subunits is 178-amino-acid long. It is primarily produced by monocytes. IL-10 is a cytokine with pleiotropic effects in immunoregulation and inflammation. It could downregulates the expression of Th1 cytokines, MHC class II antigens, and co-stimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production.
ncbi gi num :
21492460
ncbi acc num :
NP_659579.1
ncbi gb acc num :
NC_004002.1
size1 :
96 Tests
price1 :
385 USD
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.

"MyBioSource's number 1 vision is to be the world's number 1 quality reagents provider."

Our goal is to provide researchers, scientists and customers alike with a one-stop-shop for all of their reagents needs, whether it is monoclonal antibody, polyclonal antibody, recombinant protein, peptide, etc...

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