ELISA/assay

Vimentin Human ELISA Kit

MBS355298

96 Tests

440 USD

ELISA Kit

Vimentin Human ELISA Kit

[Vimentin]

[Human Vimentin]

[vimentin; Vimentin; vimentin; vimentin]

[VIM; VIM; CTRCT30]

VIME_HUMAN

Human

Store at 2-8 degree C for 6 months.

Quantitative sELISA (EIA)

For quantitative detection of Vimentin in Human serum, plasma, urine, cell culture supernatant or tissue samples.

Typical Testing Data/Standard Curve (for reference only)

Samples: Human Serum, Plasma, Urine, Cell Culture Supernatant Or Tissue Samples. Assay Type: Sandwich. Detection Range: 0.3 ng/ml-10 ng/ml

Principle of the Assay: This kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. The purified anti-Progesterone receptor antibody was pre-coated onto 96-well plates. And the HRP conjugated anti-Progesterone receptor antibody was used as detection antibodies. The standards, test samples and HRP conjugated detection antibody were added to the wells subsequently, mixed and incubated, then, unbound conjugates were washed away with wash buffer. TMB substrates (A & B) were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Progesterone receptor amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Progesterone receptor can be calculated!!Background/Introduction: The progesterone receptor (PR, also known as NR3C3), is a member of the steroid receptor superfamily, mediates the physiologic effects of progesterone. It is activated by the steroid hormone progesterone. In humans, PR is encoded by a single PGR gene residing on chromosome 11q22, it has two main forms, A and B, that differ in their molecular weight. The human progesterone receptor exists as 2 functionally distinct isoforms, PRA and PRB. PRB functions as a transcriptional activator in most cell and promoter contexts, while PRA is transcriptionally inactive and functions as a strong ligand-dependent transdominant repressor of steroid hormone receptor transcriptional activity.

62414289

NP_003371.2

NM_003380.3

P08670

53,652 Da

Alpha6-Beta4 Integrin Signaling Pathway (198807); Apoptosis Pathway (105648); Apoptotic Cleavage Of Cellular Proteins Pathway (105678); Apoptotic Execution Phase Pathway (105677); Aurora B Signaling Pathway (138080); Caspase Cascade In Apoptosis Pathway (137974); Caspase-mediated Cleavage Of Cytoskeletal Proteins Pathway (105679); Epstein-Barr Virus Infection Pathway (585562); Epstein-Barr Virus Infection Pathway (587115); MicroRNAs In Cancer Pathway (852705)

This gene encodes a member of the intermediate filament family. Intermediate filamentents, along with microtubules and actin microfilaments, make up the cytoskeleton. The protein encoded by this gene is responsible for maintaining cell shape, integrity of the cytoplasm, and stabilizing cytoskeletal interactions. It is also involved in the immune response, and controls the transport of low-density lipoprotein (LDL)-derived cholesterol from a lysosome to the site of esterification. It functions as an organizer of a number of critical proteins involved in attachment, migration, and cell signaling. Mutations in this gene causes a dominant, pulverulent cataract.[provided by RefSeq, Jun 2009]

Function: Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally. Ref.37Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2. Ref.37. Subunit structure: Homopolymer assembled from elementary dimers. Interacts with HCV core protein. Interacts with LGSN and SYNM. Interacts (via rod region) with PLEC (via CH 1 domain) . By similarity. Interacts with SLC6A4. Interacts with STK33. Interacts with LARP6. Interacts with RAB8B . By similarity. Ref.23 Ref.27 Ref.32 Ref.37 Ref.42. Subcellular location: Cytoplasm Ref.39. Tissue specificity: Highly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone-independent mammary carcinoma cell lines. Ref.12 Ref.17. Domain: The central alpha-helical coiled-coil rod region mediates elementary homodimerization. Ref.42. Post-translational modification: Filament disassembly during mitosis is promoted by phosphorylation at Ser-55 as well as by nestin . By similarity. One of the most prominent phosphoproteins in various cells of mesenchymal origin. Phosphorylation is enhanced during cell division, at which time vimentin filaments are significantly reorganized. Phosphorylation by PKN1 inhibits the formation of filaments. Phosphorylated at Ser-56 by CDK5 during neutrophil secretion in the cytoplasm. Phosphorylated by STK33. Ref.14 Ref.19 Ref.20 Ref.21 Ref.27 Ref.39. Sequence similarities: Belongs to the intermediate filament family. Sequence caution: The sequence BAB71275.1 differs from that shown. Reason: Intron retention.

96 Tests

440 USD