ELISA/assay

VEGF-R1 Human ELISA Kit

MBS355297

96 Tests

440 USD

ELISA Kit

VEGF-R1 Human ELISA Kit

[VEGF-R1]

[Human VEGF-R1]

[vascular endothelial growth factor receptor 1; Vascular endothelial growth factor receptor 1; vascular endothelial growth factor receptor 1; FLT-1; FMS-like tyrosine kinase 1; tyrosine-protein kinase receptor FLT; vascular endothelial growth factor receptor-1; Fms-related tyrosine kinase 1 (vascular endothelial growth factor/vascular permeability factor receptor); FMS-related tyrosine kinase 1; Fms-like tyrosine kinase 1; FLT-1; Tyrosine-protein kinase receptor FLT]

[VEGF-R1]

[Flt1; Flt1; VEGFR-1; Flt-1; Vegfr1; VEGFR-1; FLT-1]

VGFR1_RAT

Human

Store at 2-8 degree C for 4 months, or at -20 degree C for 8 months.

Quantitative sELISA (EIA)

For quantitative detection of VEGFR1 in human serum, plasma, body fluids, tissue lysates or cell culture supernatants.

Typical Testing Data/Standard Curve (for reference only)

Samples: human serum, plasma, body fluids, tissue lysates or cell culture supernatants. Assay Type: Sandwich (Quantitative). Detection Range: 156 pg/ml-10,000 pg/ml. Sensitivity: < 4 pg/ml

Background: Vascular endothelial growth factor receptor 1 (VEGFR1), also known as FLT1, is a protein that in humans is encoded by the FLT1 gene, which maps to 13q12. Oncogene FLT belongs to the src gene family and is related to oncogene ROS. Like other members of this family, it shows tyrosine protein kinase activity that is important for the control of cell proliferation and differentiation. The deduced 1,338-amino acid protein has a calculated molecular mass of 150.6 kD. The sequence structure of the FLT gene resembles that of the FMS gene; hence, Yoshida et al. (1987) proposed the name FLT as an acronym for FMS-like tyrosine kinase.

11276093

NP_062179.1

NM_019306.1

P53767

150,250 Da

Cytokine-cytokine Receptor Interaction Pathway (83443); Cytokine-cytokine Receptor Interaction Pathway (460); Endocytosis Pathway (102267); Endocytosis Pathway (102181); Focal Adhesion Pathway (83459); Focal Adhesion Pathway (478); HIF-1 Signaling Pathway (695233); Id Signaling Pathway (198536); PI3K-Akt Signaling Pathway (692247); PI3K-Akt Signaling Pathway (692979)

tyrosine kinase receptor for vascular endothelial growth factor; may play a role in cell proliferation and cell survival [RGD, Feb 2006]

Function: Tyrosine-protein kinase that acts as a cell-surface receptor for VEGFA, VEGFB and PGF, and plays an essential role in the development of embryonic vasculature, the regulation of angiogenesis, cell survival, cell migration, macrophage function, chemotaxis, and cancer cell invasion. May play an essential role as a negative regulator of embryonic angiogenesis by inhibiting excessive proliferation of endothelial cells. Can promote endothelial cell proliferation, survival and angiogenesis in adulthood. Its function in promoting cell proliferation seems to be cell-type specific. Promotes PGF-mediated proliferation of endothelial cells, and proliferation of some types of cancer cells, but does not promote proliferation of normal fibroblasts. Has very high affinity for VEGFA and relatively low protein kinase activity; may function as a negative regulator of VEGFA signaling by limiting the amount of free VEGFA and preventing its binding to KDR. Modulates KDR signaling by forming heterodimers with KDR. Ligand binding leads to the activation of several signaling cascades. Activation of PLCG leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate and the activation of protein kinase C. Mediates phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, leading to the activation of phosphatidylinositol kinase and the downstream signaling pathway. Mediates activation of MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling pathway, as well as of the AKT1 signaling pathway. Phosphorylates SRC, YES1 and PLCG, and may also phosphorylate CBL. Promotes phosphorylation of AKT1 and PTK2/FAK1 . By similarity. Catalytic activity: ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate. Enzyme regulation: Present in an inactive conformation in the absence of bound ligand. Binding of VEGFA, VEGFB or PGF leads to dimerization and activation by autophosphorylation on tyrosine residues . By similarity. Subunit structure: Interacts with VEGFA, VEGFB and PGF. Monomer in the absence of bound VEGFA, VEGFB or PGF. Homodimer in the presence of bound VEGFA, VEGFB and PGF. Can also form a heterodimer with KDR. Interacts (tyrosine phosphorylated) with CBL, CRK, GRB2, NCK1, PIK3R1, PLCG, PSEN1 and PTPN11. Probably interacts with PTPRB. Interacts with GNB2L1/RACK1. Identified in a complex with CBL and CD2AP . By similarity. Subcellular location: Cell membrane; Single-pass type I membrane protein. Endosome . By similarity. Note: Autophosphorylation promotes ubiquitination and endocytosis . By similarity. Domain: The second and third Ig-like C2-type (immunoglobulin-like) domains are sufficient for VEGFA binding . By similarity. Post-translational modification: N-glycosylated . By similarity.Ubiquitinated after VEGFA-mediated autophosphorylation, leading to proteolytic degradation . By similarity.Autophosphorylated on tyrosine residues upon ligand binding. Autophosphorylation occurs in trans, i.e. one subunit of the dimeric receptor phosphorylates tyrosine residues on the other subunit. Phosphorylation at Tyr-1169 is important for interaction with PLCG. Phosphorylation at Tyr-1213 is important for interaction with PIK3R1, PTPN11, GRB2, and PLCG. Phosphorylation at Tyr-1331 is important for endocytosis and for interaction with CBL, NCK1 and CRK. Is probably dephosphorylated by PTPRB . By similarity. Sequence similarities: Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.Contains 7 Ig-like C2-type (immunoglobulin-like) domains.Contains 1 protein kinase domain.

96 Tests

440 USD