product summary
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company name :
MyBioSource
product type :
ELISA/assay
product name :
HGF Human ELISA Kit
catalog :
MBS355291
quantity :
96 Tests
price :
410 USD
more info or order :
image
image 1 :
product information
catalog number :
MBS355291
products type :
ELISA Kit
products full name :
HGF Human ELISA Kit
products short name :
[HGF]
products name syn :
[Human HGF]
products gene name :
[HGF]
reactivity :
Human
storage stability :
Store at 2-8 degree C for 4 months, or at -20 degree C for 8 months.
tested application :
Quantitative sELISA (EIA)
app notes :
For quantitative detection of HGF in human serum, plasma, body fluids, tissue lysates or cell culture supernatants.
image1 heading :
Typical Testing Data/Standard Curve
other info1 :
Samples: Human serum, plasma, body fluids, tissue lysates or cell culture supernatants. Assay Type: Quantitative Sandwich. Sensitivity: < 5 pg/ml
products description :
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-HGF polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-HGF polyclonal antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the HGF amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of HGF can be calculated. Background/Introduction: Hepatocyte growth factor/scatter factor (HGF/SF) is a paracrine cellular growth, motility and morphogenic factor. It consists of 2 chains, heavy and light, with molecular mass of 54 to 65 kD and 31.5 and 34.5 kD, respectively. It is secreted by mesenchymal cells and targets and acts primarily upon epithelial cells and endothelial cells, but also acts on haemopoietic progenitor cells. It has been shown to have a major role in embryonic organ development, in adult organ regeneration and in wound healing. Human HGF plasmid DNA therapy of cardiomyocytes is being examined as a potential treatment for coronary artery disease as well as treatment for the damage that occurs to the heart after myocardial infarction. It may further play a role as an indicator for prognosis of chronicity for Chikungunya virus induced arthralgia.
size1 :
96 Tests
price1 :
410 USD
more info or order :
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.
"MyBioSource's number 1 vision is to be the world's number 1 quality reagents provider."
Our goal is to provide researchers, scientists and customers alike with a one-stop-shop for all of their reagents needs, whether it is monoclonal antibody, polyclonal antibody, recombinant protein, peptide, etc...
"MyBioSource offers the best products at unbeatable prices."
Please spend a few minutes to browse our online catalogs and see the wide range of products available. We ship our products through our shipping/distribution facility in San Diego, California, USA.
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