ELISA/assay

NGF Human ELISA Kit

MBS355238

96 Tests

415 USD

ELISA Kit

NGF Human ELISA Kit

NGF

Human NGF

NGF protein; Beta-nerve growth factor; beta-nerve growth factor; nerve growth factor, beta subunit; nerve growth factor (beta polypeptide)

NGF

NGF; NGF; NGFB; HSAN5; Beta-NGF; NGFB; Beta-NGF

NGF_HUMAN

Human

Store at 2-8 degree C for 4 months, or at -20 degree C for 8 months.

Quantitative sELISA (EIA)

For quantitative detection of NT-3 in Human serum, plasma, body fluids, tissue lysates or cell culture supernatants.

Assay Type: Sandwich. Detection Range: 15.6 pg/ml-1000 pg/ml. Sensitivity: < 1 pg/ml

Principle of the assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-NGF polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-NGF polyclonal antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the NGF amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of NGF can be calculated. Background: Nerve growth factor (NGF) is a polypeptide involved in the regulation of growth and differentiation of sympathetic and certain sensory neurons. NGF consists of 3 types of subunits, alpha, beta and gamma, which specifically interact to form a 7S, approximately 130, 000-molecular weight complex. It is mainly expressed in Sertoli and interstitial cells. The proforms of NGF are secreted and cleaved extracellularly by the serine protease plasmin and by selective matrix metalloproteinases. NGF is important for the growth, maintenance, and survival of certain target neurons (nerve cells). It also functions as a signaling molecule.

34192369

AAH32517.2

P01138

26,959 Da

ARMS-mediated Activation Pathway (106466); Activation Of TRKA Receptors Pathway (106456); Apoptosis Pathway (83060); Apoptosis Pathway (470); Axonal Growth Stimulation Pathway (106453); BDNF Signaling Pathway (712093); Cell Death Signalling Via NRAGE, NRIF And NADE Pathway (106443); Ceramide Signalling Pathway (106451); Frs2-mediated Activation Pathway (106465); Id Signaling Pathway (198871)

This gene is a member of the NGF-beta family and encodes a secreted protein which homodimerizes and is incorporated into a larger complex. This protein has nerve growth stimulating activity and the complex is involved in the regulation of growth and the differentiation of sympathetic and certain sensory neurons. Mutations in this gene have been associated with hereditary sensory and autonomic neuropathy, type 5 (HSAN5), and dysregulation of this gene's expression is associated with allergic rhinitis. [provided by RefSeq, Jul 2008]

Function: Nerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems. Extracellular ligand for the NTRK1 and NGFR receptors, activates cellular signaling cascades through those receptor tyrosine kinase to regulate neuronal proliferation, differentiation and survival. Subunit structure: Homodimer. Subcellular location: Secreted. Involvement in disease: Hereditary sensory and autonomic neuropathy 5 (HSAN5) [MIM:608654]: A form of hereditary sensory and autonomic neuropathy, a genetically and clinically heterogeneous group of disorders characterized by degeneration of dorsal root and autonomic ganglion cells, and by sensory and/or autonomic abnormalities. HSAN5 patients manifest loss of pain perception and impaired temperature sensitivity, ulcers, and in some cases self-mutilation. The autonomic involvement is variable.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.17 Ref.18 Ref.19. Sequence similarities: Belongs to the NGF-beta family. Sequence caution: The sequence AAH32517.2 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

96 Tests

415 USD