product summary
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company name :
MyBioSource
product type :
ELISA/assay
product name :
IL-10 Rat ELISA Kit
catalog :
MBS355232
quantity :
96 Tests
price :
385 USD
more info or order :
MyBioSource product webpage
image
image 1 :
MyBioSource MBS355232 image 1
product information
catalog number :
MBS355232
products type :
ELISA Kit
products full name :
IL-10 Rat ELISA Kit
products short name :
[IL-10]
products name syn :
[Rat IL-10]
other names :
[IL-10; IL-10]
products gene name :
[IL-10]
other gene names :
[SPPV_03]
reactivity :
Rat
specificity :
This assay has high sensitivity and excellent specificity for detection of humanin. No significant cross-reactivity or interference between humanin and analogues was observed.
storage stability :
Store at 2-8 degree C for 4 months, or at -20 degree C for 8 months.
tested application :
Quantitative sELISA (EIA)
app notes :
For quantitative detection of IL-10 in rat serum, body fluids, tissue lysates or cell culture supernatants.
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Serum, Plasma, Tissue Homogenates And Other Biological Fluids. Assay Type: Sandwich. Detection Range: 31.25-2000pg/ml. Sensitivity: <18.75pg/ml
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level humanin were tested 20 times on one plate, respectively. Intra-Assay: CV<8%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level humanin were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
products description :
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Antihumanin antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-humanin antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the humanin amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of humanin can be calculated.
ncbi gi num :
21492460
ncbi acc num :
NP_659579.1
ncbi gb acc num :
NC_004002.1
size1 :
96 Tests
price1 :
385 USD
more info or order :
MyBioSource product webpage
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.

"MyBioSource's number 1 vision is to be the world's number 1 quality reagents provider."

Our goal is to provide researchers, scientists and customers alike with a one-stop-shop for all of their reagents needs, whether it is monoclonal antibody, polyclonal antibody, recombinant protein, peptide, etc...

"MyBioSource offers the best products at unbeatable prices."

Please spend a few minutes to browse our online catalogs and see the wide range of products available. We ship our products through our shipping/distribution facility in San Diego, California, USA.

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