This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
MyBioSource
product type :
ELISA/assay
product name :
TGFbeta1 Mouse ELISA Kit
catalog :
MBS355229
quantity :
96 Tests
price :
385 USD
product information
catalog number :
MBS355229
products type :
ELISA Kit
products full name :
TGFbeta1 Mouse ELISA Kit
products short name :
[TGFbeta1]
products name syn :
[Mouse TGFbeta1]
products gene name :
[TGFbeta1]
reactivity :
Mouse
storage stability :
Store at 2-8 degree C for 4 months, or at -20 degree C for 8 months.
tested application :
Quantitative sELISA (EIA)
app notes :
For quantitative detection of TGFbeta1 in mouse serum, body fluids, tissue lysates or cell culture supernatants.
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: mouse serum, body fluids, tissue lysates or cell culture supernatants. Assay Type: Quantitative Sandwich. Detection Range: 15.6 pg/ml-1000 pg/ml. Sensitivity: < 2 pg/ml
products description :
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- TGFbeta1 polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- TGFbeta1 polyclonal antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the TGFbeta1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of TGFbeta1 can be calculated. Background: Transforming growth factor beta 1 or TGFbeta1 is a polypeptide member of the transforming growth factor beta superfamily of cytokines. In humans, TGFbeta1 is encoded by the TGFB1 gene. This gene contains 7 exons and very large introns, maps to 19q13.1-q13.3. TGFbeta1 acts synergistically with TGFA in inducing transformation. It also acts as a negative autocrine growth factor. The TGFB1 is directly involved in the pathogenesis of bone marrow reticulin fibrosis in hairy cell leukemia. The expression of TGFB1 in the early stages of DMD may be critical in initiating muscle fibrosis, and suggested that antifibrosis treatment might slow progression of the disease, increasing the utility of gene therapy.
size1 :
96 Tests
price1 :
385 USD
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.

"MyBioSource's number 1 vision is to be the world's number 1 quality reagents provider."

Our goal is to provide researchers, scientists and customers alike with a one-stop-shop for all of their reagents needs, whether it is monoclonal antibody, polyclonal antibody, recombinant protein, peptide, etc...

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