antibody

Anti-14-3-3 beta / alpha antibody

MBS355189

0.1 mg

285 USD

polyclonal

rabbit

nonconjugated

human, mouse, rat

Antibody

Anti-14-3-3 beta / alpha antibody

14-3-3 beta / alpha

Polyclonal Anti- 14-3-3 beta / alpha

14-3-3 protein beta/alpha; 14-3-3 protein beta/alpha; 14-3-3 protein beta/alpha; 14-3-3 alpha; protein 1054; brain protein 14-3-3, beta isoform; protein kinase C inhibitor protein 1; protein kinase C inhibitor protein-1; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, alpha polypeptide; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta polypeptide; Protein 1054; Protein kinase C inhibitor protein 1

YWHAB; YWHAB; HS1; GW128; YWHAA; KCIP-1; KCIP-1

1433B_HUMAN

Polyclonal

IgG

Rabbit

Human, mouse, rat

Immunogen affinity purified

Liquid

Store at 4 degree C after thawing (1 week). Aliquot and store at -20 degree C for long term (at least one year). Avoid repeated freeze and thaw cycles.

Western Blot (WB)

WB (1:1000)

Immunogen: A synthetic peptide (conjugated with KLH) corresponding to the C-terminal residues of mouse 14-3-3 b/a.

Storage Buffer: In TBS (pH7.4), 0.5% BSA, 40% Glycerol and 0.05% Sodium Azide. Storage Buffer: In TBS (pH7.4), 0.5% BSA, 40% Glycerol and 0.05% Sodium Azide.

14-3-3 proteins are a family of conserved regulatory molecules expressed in all eukaryotic cells. They have the ability to bind a multitude of functionally diverse signaling proteins, including kinases, phosphatases, and transmembrane receptors. More than 100 signaling proteins have been reported as 14-3-3 ligands, 14-3-3 b/a is one of them. They play important roles in a wide range of vital regulatory processes, such as mitogenic signal transduction, apoptotic cell death, and cell cycle control. Elevated amounts of 14-3-3 proteins are found in the cerebrospinal fluid of patients with Creutzfeldt-Jakob disease.[1]

1. Takahashi H, Iwata T, Kitagawa Y et al. (November 1999). "Increased levels of epsilon and gamma isoforms of 14-3-3 proteins in cerebrospinal fluid in patients with Creutzfeldt-Jakob disease". Clin. Diagn. Lab. Immunol. 6 (6): 983-5.

21328448

NP_647539.1

NM_139323.3

P31946

28,082 Da

ARMS-mediated Activation Pathway 106466!!Activation Of BAD And Translocation To Mitochondria Pathway 105659!!Activation Of BH3-only Proteins Pathway 105658!!Adaptive Immune System Pathway 366160!!Alpha6-Beta4 Integrin Signaling Pathway 198807!!Apoptosis Pathway 105648!!Axon Guidance Pathway 105688!!Calcium Regulation In The Cardiac Cell Pathway 198906!!Cell Cycle Pathway 198811!!Cell Cycle Pathway 83054

This gene encodes a protein belonging to the 14-3-3 family of proteins, members of which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals. The encoded protein has been shown to interact with RAF1 and CDC25 phosphatases, suggesting that it may play a role in linking mitogenic signaling and the cell cycle machinery. Two transcript variants, which encode the same protein, have been identified for this gene. [provided by RefSeq, Jul 2008]

Function: Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negative regulator of osteogenesis. Blocks the nuclear translocation of the phosphorylated form (by AKT1) of SRPK2 and antagonizes its stimulatory effect on cyclin D1 expression resulting in blockage of neuronal apoptosis elicited by SRPK2. Ref.13 Ref.15. Subunit structure: Homodimer. Interacts with SAMSN1 and PRKCE . By similarity. Interacts with SSH1 and TORC2/CRTC2. Interacts with ABL1; the interaction results in cytoplasmic location of ABL1 and inhibition of cABL-mediated apoptosis. Interacts with ROR2 (dimer); the interaction results in phosphorylation of YWHAB on tyrosine residues. Interacts with GAB2 and YAP1 (phosphorylated form). Interacts with the phosphorylated (by AKT1) form of SRPK2. Interacts with PKA-phosphorylated AANAT. Subcellular location: Cytoplasm. Melanosome. Note: Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Ref.11. Post-translational modification: The alpha, brain-specific form differs from the beta form in being phosphorylated . By similarity. Phosphorylated on Ser-60 by protein kinase C delta type catalytic subunit in a sphingosine-dependent fashion . By similarity. Ref.13Isoform Short contains a N-acetylmethionine at position 1 . By similarity. Sequence similarities: Belongs to the 14-3-3 family.

0.1 mg

285 USD