catalog number :
MBS355117
products full name :
Anti-Lactate dehydrogenase A, LDH-A antibody
products short name :
[Lactate dehydrogenase A, LDH-A]
products name syn :
[Monoclonal Anti- Lactate dehydrogenase A; LDH-A]
other names :
[lactate dehydrogenase A, partial]
products gene name :
[LDH-A]
reactivity :
Human, mouse
purity :
Protein A or Protein G purified
form :
Liquid; In TBS (pH7.4), 0.5% BSA, 40% Glycerol and 0.05% Sodium Azide.
storage stability :
Store at 4°C after thawing (1 week). Aliquot and store at -20°C for long term (at least one year). Avoid repeated freeze and thaw cycles.
tested application :
Western Blot (WB), Immunohistochemistry (IHC) Paraffin. Other applications have not been tested.
app notes :
Western Blot 1:500. Immunohistochemistry-Paraffin 1:50. The optimal dilutions should be determined by end user.
image1 heading :
Western Blot (WB)
other info1 :
Samples: Tissue Extracts, Cell Lysate, Cell Culture Media And Other Biological Fluids Samples. Detection Range: 4 umol/L-400 umol/L
products description :
Background/Introduction: Isocitrate lyase (EC 4.1.3.1), or ICL, is an enzyme in the glyoxylate cycle that catalyzes the cleavage of isocitrate to succinate and glyoxylate. Together with malate synthase, it bypasses the two decarboxylation steps of the tricarboxylic acid cycle (TCA cycle) and is used by bacteria, fungi, and plants. The assay is initiated with the enzymatic decomposition of the Isocitric acid by Isocitrate lyase. The enzyme catalysed reaction product NADH can be measured at a colorimetric readout at 340 nm.
products references :
1. Chung, F.-Z., Tsujibo, H., Bhattacharyya, U., Sharief, F. S., Li, S. S.-L. Genomic organization of human lactate dehydrogenase-A gene. Biochem. J. 231: 537-541, 1985. 2. Tsujibo, H., Tiano, H. F., Li, S. S.-L. Nucleotide sequences of the cDNA and an intronless pseudogene for human lactate dehydrogenase-A isozyme. Europ. J. Biochem. 147: 9-15, 1985.