antibody

Mouse anti-Human Insulin B chain Monoclonal Antibody

MBS355071

0.1 mg

275 USD

monoclonal

mouse

nonconjugated

[N42]

ELISA, immunohistochemistry, enzyme immunoassay, immunohistochemistry - paraffin section

Antibody

Mouse anti-Human Insulin B chain Monoclonal Antibody

[Insulin B chain]

[Monoclonal Anti- Insulin B chain]

Monoclonal

IgG1

[N42]

Mouse

Human, mouse, rat

Protein A or Protein G purified

Liquid

Store at 4°C after thawing (1 week). Aliquot and store at -20°C for long term (at least one year). Avoid repeated freeze and thaw cycles

ELISA (EIA), Immunohistochemistry (IHC)-Paraffin

ELISA (1:10000); Immunohistochemistry (IHC-P) (1:100-1000)

Background/Introduction: Indoleamine 2,3-Dioxygenase 1 (IDO1, EC 1.13.11.52) is a cytoplasmic hemoprotein that oxidizes tryptophan yielding N-formylkynurenine (NFK). In mammals, this reaction is the first and rate limiting step in the kynurenine catabolic pathway. IDO1 activity is low under normal physiological conditions, but is dramatically upregulated by proinflammatory cytokines such as interferon-gamma. This short-term activation of IDO1 occurs as part of the innate immune response and helps to inhibit the growth of pathogens and parasites. IDO1 activation can also promote host immune tolerance by exerting an immunosuppressive effect. IDO1 expression by tumor cells plays a substantial role in tumor immune tolerance, aiding tumors in evading detection and destruction. Chronic induction of IDO1 expression has been found in cancer patients increased IDO1 activity is correlated with negative prognosis. IDO1 has thus become an attractive pharmacological target for development of novel antineoplastics and adjuvants to increase the efficacy of conventional chemotherapy. IDO1 Activity Assay Kit enables IDO1 activity to be easily determined in mammalian tissues and cell lines. The assay uses a fluorogenic developer that selectively reacts with NFK to produce a highly fluorescent product (Ex/Em = 402/488 nm), ensuring a high signal-to-background ratio. The kit also includes a highly selective IDO1 inhibitor for verification of enzyme activity in biological matrices. The assay has a simple nowash protocol, is high-throughput adaptable and can detect down to 0.2 mU of IDO1 activity or 200 pmole NFK.

1. Steiner, D. F., Oyer, P. E. The biosynthesis of insulin and a probable precursor of insulin by a human islet cell adenoma. Proc. Nat. Acad. Sci. 57: 473-480, 1967. 2. Dandona, P., Aljada, A., Mohanty, P., Ghanim, H., Hamouda, W., Assian, E., Ahmad, S. Insulin inhibits intranuclear nuclear factor kappa-B and stimulates I-kappa-B in mononuclear cells in obese subjects: evidence for an anti-inflammatory effect? J. Clin. Endocr. Metab. 86: 3257-3265, 2001. 3. Frosig, C., Rose, A. J., Treebak, J. T., Kiens, B., Richter, E. A., Wojtaszewski, J. F. P. Effects of endurance exercise training on insulin signaling in human skeletal muscle: interactions at the level of phosphatidylinositol 3-kinase, Akt, and AS160. Diabetes 56: 2093-2102, 2007.

0.1 mg

275 USD