antibody

Anti-Polycomb complex protein BMI-1 antibody

MBS355063

0.1 mg

285 USD

polyclonal

mouse

nonconjugated

P9

western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Antibody

Anti-Polycomb complex protein BMI-1 antibody

Polycomb complex protein BMI-1

Monoclonal Anti- Polycomb complex protein BMI-1

polycomb complex protein BMI-1; Polycomb complex protein BMI-1; polycomb complex protein BMI-1; flvi-2/bmi-1; ring finger protein 51; polycomb group protein Bmi1; polycomb group RING finger protein 4; B lymphoma Mo-MLV insertion region 1 homolog; murine leukemia viral (bmi-1) oncogene homolog; BMI1 polycomb ring finger oncogene; Polycomb group RING finger protein 4; RING finger protein 51

BMI1; BMI1; PCGF4; RNF51; FLVI2/BMI1; PCGF4; RNF51

BMI1_HUMAN

Polyclonal

IgG2a

P9

Mouse

Human, mouse

Protein A or Protein G purified

Liquid

Store at 4 degree C after thawing (1 week). Aliquot and store at -20 degree C for long term (at least one year). Avoid repeated freeze and thaw cycles

Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Immunocytochemistry (ICC)

WB (1:1000-5000); IHC-P (1:200); ICC (1:500). Other applications have not been tested the optimal dilutions should be determined by end user.

Background: BMI1 has been reported as an oncogene by regulating p16 and p19, which are cell cycle inhibitor genes. BMI1 knockout in mice results in defects in hematopoiesis, skeletal patterning, neurological functions, and development of the cerebellum. It is a component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development.[1] BMI1 is necessary for efficient self-renewing cell divisions of adult hematopoietic stem cells as well as adult peripheral and central nervous system neural stem cells.[2] BMI1 is also thought to inhibit ageing in neurons through the suppression of p53.[3]

1. Buchwald G., van der Stoop P., Weichenrieder O., Perrakis A., van Lohuizen M., Sixma T.K. "Structure and E3-ligase activity of the Ring-Ring complex of polycomb proteins Bmi1 and Ring1b." EMBO J. 25:2465-2474(2006) 2. Park IK, Morrison SJ, Clarke MF (January 2004). "Bmi1, stem cells, and senescence regulation". J. Clin. Invest. 113 (2): 175-9. 3. Chatoo W, Abdouh M, David J, Champagne MP, Ferreira J, Rodier F, Bernier G (2009).

27883842

NP_005171.4

NM_005180.8

P35226

36,949 Da

MicroRNAs In Cancer Pathway (852705); MicroRNAs In Cancer Pathway (852928); Senescence And Autophagy Pathway (198780); Transcriptional Misregulation In Cancer Pathway (523016); Transcriptional Misregulation In Cancer Pathway (522987); Validated Targets Of C-MYC Transcriptional Activation Pathway (169351)

This gene encodes a ring finger protein that is major component of the polycomb group complex 1 (PRC1). This complex functions through chromatin remodeling as an essential epigenetic repressor of multiple regulatory genes involved in embryonic development and self-renewal in somatic stem cells. This protein also plays a central role in DNA damage repair. This gene is an oncogene and aberrant expression is associated with numerous cancers and is associated with resistance to certain chemotherapies. A pseudogene of this gene is found on chromosome X. Read-through transcription also exists between this gene and the upstream COMM domain containing 3 (COMMD3) gene. [provided by RefSeq, Sep 2015]

Function: Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. In the PRC1 complex, it is required to stimulate the E3 ubiquitin-protein ligase activity of RNF2/RING2. Ref.11 Ref.13 Ref.16. Subunit structure: Component of a PRC1-like complex. Interacts with RING1 and RING2 . By similarity. Interacts vwith CBX7 and CBX8. Interacts with SPOP. Part of a complex consisting of BMI1, CUL3 and SPOP. Interacts with E4F1. Ref.7 Ref.8 Ref.9 Ref.10 Ref.12 Ref.13 Ref.14 Ref.15 Ref.16. Subcellular location: Nucleus. Cytoplasm Ref.13 Ref.15. Post-translational modification: Monoubiquitinated . By similarity. May be polyubiquitinated; which does not lead to proteasomal degradation. Miscellaneous: The hPRC-H complex purification reported by Ref.9 probably presents a mixture of different PRC1-like complexes. Sequence similarities: Contains 1 RING-type zinc finger.

0.1 mg

285 USD