antibody

Anti-Insulin (B chain) antibody

MBS355059

0.1 mg

275 USD

polyclonal

rabbit

nonconjugated

ELISA, flow cytometry, FACS, enzyme immunoassay, immunohistochemistry - paraffin section

Antibody

Anti-Insulin (B chain) antibody

[Insulin (B chain)]

[Polyclonal Anti- Insulin (B chain)]

Polyclonal

IgG

Rabbit

Human

Immunogen affinity purified

Liquid; TBS (pH7.4), 0.5% BSA, 40% Glycerol and 0.05% Sodium Azide

Store at 4 degree C after thawing (1 week). Aliquot and store at -20 degree C for long term (at least one year). Avoid repeated freeze and thaw cycles

ELISA (EIA), Immunohistochemistry - Paraffin (IHC-P), Flow Cytometry (FC/FACS)

Recommended Application: . ELISA (1:5000). IHC-P (1:100-400). FC/FACS (1:50-100). Other applications have not been tested. The optimal dilutions should be determined by end user.

Immunogen: A synthetic peptide (conjugated with KLH) corresponding to the region of human Insulin (B chain).

Insulin, synthesized by the beta cells of the islets of Langerhans, consists of 2 dissimilar polypeptide chains, A and B, which are linked by 2 disulfide bonds. The human insulin gene contains 3 exons; exon 2 encodes the signal peptide, the B chain, and part of the C peptide, while exon 3 encodes the remainder of the C peptide and the A chain.1 Insulin has a potent acute antiinflammatory effect, including a reduction in intranuclear NF-kappa-B, an increase in IKB, and decreases in the generation of reactive oxygen species.2 It causes cells in the liver, muscle, and fat tissue to take up glucose from the blood, storing it as glycogen inside these tissues. And improved insulin-stimulated glucose uptake after endurance training results from hemodynamic adaptations as well as increased cellular protein content of individual insulin signaling components and molecules involved in glucose transport and metabolism.3

1. Steiner, D. F., Oyer, P. E. The biosynthesis of insulin and a probable precursor of insulin by a human islet cell adenoma. Proc. Nat. Acad. Sci. 57: 473-480, 1967. 2. Dandona, P., Aljada, A., Mohanty, P., Ghanim, H., Hamouda, W., Assian, E., Ahmad, S. Insulin inhibits intranuclear nuclear factor kappa-B and stimulates I-kappa-B in mononuclear cells in obese subjects: evidence for an anti-inflammatory effect? J. Clin. Endocr. Metab. 86: 3257-3265, 2001. 3. Frosig, C., Rose, A. J., Treebak, J. T., Kiens, B., Richter, E. A., Wojtaszewski, J. F. P. Effects of endurance exercise training on insulin signaling in human skeletal muscle: interactions at the level of phosphatidylinositol 3-kinase, Akt, and AS160. Diabetes 56: 2093-2102, 2007.

0.1 mg

275 USD