product summary
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company name :
MyBioSource
product type :
antibody
product name :
His tag(HRP Conjugated) tag antibody
catalog :
MBS355032
quantity :
0.1 mg
price :
250 USD
clonality :
monoclonal
host :
mouse
conjugate :
HRP
application :
western blot
more info or order :
MyBioSource product webpage
product information
catalog number :
MBS355032
products type :
Antibody
products full name :
His tag(HRP Conjugated) tag antibody
products short name :
[His Tag (HRP Conjugated)]
products name syn :
[Monoclonal Anti-His Tag (HRP Conjugated)]
clonality :
m
isotype :
IgG1
host :
Mouse
reactivity :
All species
specificity :
Detect over-expressed or recombinant proteins containing the 6 × His epitope tag.
form :
Liquid (1mg/ml)
storage stability :
Store at -20 degree C for 1 year. Or aliquot and store at -80 degree C for long term. Avoid repeated freeze and thaw cycles
tested application :
Western Blot (WB)
app notes :
Western Blot (1:1000-10000). Other applications have not been tested. The optimal dilutions should be determined by end user.
products categories :
Tag & Loading Control Antibodies
products description :
Background/Introduction: A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein. It is also known as hexa histidine-tag, 6xHis-tag. Polyhistidine-tags are often used for affinity purification of polyhistidine-tagged recombinant proteins expressed in Escherichia coli and other prokaryotic expression systems. Affinity purification using a polyhistidine-tag usually results in relatively pure protein when the recombinant protein is expressed in prokaryotic organisms. Depending on downstream applications including the purification of protein complexes to study protein interactions, purification from higher organisms such as yeasts or other eukaryotes may require a tandem affinity purification using two tags to yield higher purity. The most common polyhistidine tags are formed of six histidine (6xHis tag) residues - preceded by Methionine - which are added at the C-terminal or N-terminal of the protein of interest.
products references :
1. Hengen, P (1995). "Purification of His-Tag fusion proteins from Escherichia coli". Trends in Biochemical Sciences 20 (7): 285-6. 2. Gavin, AC; Bösche, M; Krause, R; Grandi, P; Marzioch, M; Bauer, A; Schultz, J; Rick, JM et al. (2002). "Functional organization of the yeast proteome by systematic analysis of protein complexes". Nature 415 (6868): 141-7.
size1 :
0.1 mg
price1 :
250 USD
more info or order :
MyBioSource product webpage
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.

"MyBioSource's number 1 vision is to be the world's number 1 quality reagents provider."

Our goal is to provide researchers, scientists and customers alike with a one-stop-shop for all of their reagents needs, whether it is monoclonal antibody, polyclonal antibody, recombinant protein, peptide, etc...

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Please spend a few minutes to browse our online catalogs and see the wide range of products available. We ship our products through our shipping/distribution facility in San Diego, California, USA.

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