product summary
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company name :
MyBioSource
product type :
antibody
product name :
PTH [1-34] (Human) IgG fraction
catalog :
MBS352250
quantity :
0.1 mL
price :
610 USD
clonality :
polyclonal
host :
unidentified
conjugate :
nonconjugated
application :
western blot
more info or order :
product information
catalog number :
MBS352250
products type :
Antibody
products full name :
PTH [1-34] (Human) IgG fraction
products short name :
[PTH [1-34] (Human) IgG fraction]
products name syn :
[Parathyroid Hormone [1-34]; PTH [1-34] (Human); IgG Fraction of Rabbit Anti-hPTH (1-34) Serum]
other names :
[pth; Peptidyl-tRNA hydrolase]
other gene names :
[pth]
uniprot entry name :
PTH_STACT
clonality :
Polyclonal
host :
unknown
specificity :
Polypeptide ::: % Cross Reactivity . PTH (1-34) (human) ::: 100. PTH (1-84) (human) ::: 100. PTH (1-84) (bovine) ::: 80. PTHrp (1-35) ::: < 0.1. ANP ::::::: 0. ACTH (1-39) (human)::::::: 0. VIP:::::: 0
form :
Each vial contains 1.6 mg of purified IgG.
tested application :
Immunocytochemical Staining, Western Blot
app notes :
Immunocytochemistry: This IgG fraction has been found to stain formaldehyde fixed sections known to express PTH at a concentration of 25 ug/ml. Western Blot: 1. After SDS-PAGE on a 15% gel and electrophoretic transfer to PVDF membrane. block the membrane overnight with 2% normal goat serum in TBS/Tween-20 buffer. 2. Wash x 2 with TBS/Tween-20. 3. Apply the IgG fraction after dilution to at least 25 ug/ml (Note: higher dilutions may be needed). Use 2% normal goat serum in TBS/Tween-20 as buffer for the primary antibody. Let the primary antibody bind for 2-4 hours. 4. Wash x 3 with TBS/Tween-20. 5. Apply affinity purified HRP-goat anti-rabbit IgG antiserum diluted 1:2500 (dilution may vary depending upon supplier) in 2% normal goat serum in TBS/Tween-20. Incubate 1-2 hours. Note: greater sensitivity may be achieved using ABC techniques. 6. Wash x 4 for 5 minute/wash with TBS/Tween-20. 7. Develop color using the enhanced DAB reaction.
other info1 :
Notes: Each vial contains 1.6 mg of purified IgG.
products categories :
Histochemical Grade Antibodies
products description :
This IgG fraction was prepared by precipitation, dialysis and chromatography from an antiserum that was raised in a rabbit which was immunized with hPTH(1-34) covalently attached onto a carrier protein. The antiserum has been shown to be specific for hPTH(1-34) and was characterized by western blotting, ELISA, RIA and cell staining techniques. This IgG fraction is suitable for immunocytochemical and western immunoblotting detection of hPTH. Dilute the lyophilized antibody with 0.1 ml of 10 mg/ml BSA in PBS for the equivalent concentration in whole antiserum, or with additional buffer for more dilute antibody.
ncbi gi num :
222420251
uniprot acc num :
B9DLD9
ncbi mol weight :
21,544 Da
ncbi pathways :
Focal Adhesion Pathway (83067); Focal Adhesion Pathway (478); MAPK Signaling Pathway (83048); MAPK Signaling Pathway (456); Salmonella Infection Pathway (375172); Salmonella Infection Pathway (375149)
uniprot summary :
Function: The natural substrate for this enzyme may be peptidyl-tRNAs which drop off the ribosome during protein synthesis. By similarity. HAMAP MF_00083. Catalytic activity: N-substituted aminoacyl-tRNA + H2O = N-substituted amino acid + tRNA. HAMAP MF_00083. Subunit structure: Monomer. By similarity. HAMAP MF_00083. Subcellular location: Cytoplasm. By similarity HAMAP MF_00083. Sequence similarities: Belongs to the PTH family.
size1 :
0.1 mL
price1 :
610 USD
more info or order :
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.
"MyBioSource's number 1 vision is to be the world's number 1 quality reagents provider."
Our goal is to provide researchers, scientists and customers alike with a one-stop-shop for all of their reagents needs, whether it is monoclonal antibody, polyclonal antibody, recombinant protein, peptide, etc...
"MyBioSource offers the best products at unbeatable prices."
Please spend a few minutes to browse our online catalogs and see the wide range of products available. We ship our products through our shipping/distribution facility in San Diego, California, USA.
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