antibody

Rabbit Anti-Nicotinic beta 2 Receptor Sera

MBS352182

0.1 mL

300 USD

polyclonal

rabbit

nonconjugated

Antibody

Rabbit Anti-Nicotinic beta 2 Receptor Sera

Nicotinic Beta 2 receptor

carboxyl terminal; Nicotinic b 2 Receptor

neuronal nicotinic acetylcholine receptor beta 2; Neuronal acetylcholine receptor subunit beta-2; neuronal acetylcholine receptor subunit beta-2; OTTHUMP00000035374; neuronal nicotinic acetylcholine receptor beta 2; cholinergic receptor, nicotinic, beta polypeptide 2 (neuronal); cholinergic receptor, nicotinic, beta 2 (neuronal)

CHRNB2; CHRNB2; EFNL3; nAChRB2

ACHB2_HUMAN

Polyclonal

Rabbit

Polypeptide ::: % Cross Reactivity . Nicotinic beta 2 receptor (493-502) ::: 100. Nicotinic beta 2 receptor ::: ~90

Antiserum Specificity

Immunocytochemical Staining, Western Immunoblot, Western Blot

Immunocytochemistry: The pooled antisera have been found to stain PLP fixed cells known to express the nicotinic beta 2 receptor using ABC techniques at a 1:200 dilution. Western Blot: Western immunoblots using tissue homogenates have been successful at a 1:400 dilution and yield a single band at 60 kDa. Western Blot Protocol: 1. After SDS-PAGE (on either 4-15% gradient gels or single percentage gels. such at 12% gels) and electrophoretic transfer to PVDF membrane. block the membrane overnight with 4% normal goat serum using TBS/Tween-20 buffer. 2. Wash x 2 with TBS/Tween-20. 3. Apply the antibody after preparing a 1:400 dilution (Note: higher dilutions may be needed). Use 2% normal goat serum in TBS/Tween-20. Let the primary antibody bind for 2-4 hours. 4. Wash x 3 with TBS/Tween-20. 5. Apply affinity purified HRP-goat anti-rabbit IgG antiserum diluted 1:2500 (dilution may vary depending upon supplier) in 2% normal goat serum in TBS/Tween-20. Incubate 1-2 hours. Note: greater sensitivity may be achieved using ABC techniques. 6. Wash for 5 minutes x 4 in TBS/Tween-20. 7. Develop color using the enhanced DAB reaction.

Histochemical Grade Antibodies; Nicotinic Receptor Antibodies

These antisera were raised in rabbits which were immunized with a peptide analogue of the carboxyl terminal of the nicotinic ?2 receptor (residues #493-502) attached onto a carrier protein. The antisera have been shown to be specific for the COOH terminal of the nicotinic ?2 receptor. have been characterized by western blotting. ELISA. and cell staining techniques. and are suitable for immunocytochemical and western immunoblotting detection of the receptor. Dilute the lyophilized antibody with 0.1 ml of 10 mg/ml BSA in PBS for the equivalent of whole antiserum. or with additional buffer for more dilute antibody

5579088

P17787

57,019 Da

Acetylcholine Binding And Downstream Events Pathway (106535); Activation Of Nicotinic Acetylcholine Receptors Pathway (161027); Cholinergic Synapse Pathway (217716); Highly Calcium Permeable Nicotinic Acetylcholine Receptors Pathway (161029); Highly Calcium Permeable Postsynaptic Nicotinic Acetylcholine Receptors Pathway (161032); Highly Sodium Permeable Acetylcholine Nicotinic Receptors Pathway (161030); Neuroactive Ligand-receptor Interaction Pathway (83053); Neuroactive Ligand-receptor Interaction Pathway (462); Neuroransmitter Receptor Binding And Downstream Transmission In The Postsynaptic Cell Pathway (106534); Nicotine Activity On Dopaminergic Neurons Pathway (198858)

Neuronal acetylcholine receptors are homo- or heteropentameric complexes composed of homologous alpha and beta subunits. They belong to a superfamily of ligand-gated ion channels which allow the flow of sodium and potassium across the plasma membrane in response to ligands such as acetylcholine and nicotine. This gene encodes one of several beta subunits. Mutations in this gene are associated with autosomal dominant nocturnal frontal lobe epilepsy. [provided by RefSeq]

Function: After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane. Subunit structure: Neuronal AChR is composed of two different types of subunits: alpha and beta. Beta-2 subunit can be combined to alpha-2, alpha-3 or alpha-4 to give rise to functional receptors. Interacts with RIC3; which is required for proper folding and assembly. Ref.7. Subcellular location: Cell junction synapse postsynaptic cell membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein. Involvement in disease: Defects in CHRNB2 are the cause of nocturnal frontal lobe epilepsy type 3 (ENFL3) [. MIM:605375]. ENFL3 is an autosomal dominant epilepsy characterized by nocturnal seizures with hyperkinetic automatisms and poorly organized stereotyped movements. Ref.8 Ref.9. Sequence similarities: Belongs to the ligand-gated ion channel (TC 1.A.9) family. Acetylcholine receptor (TC 1.A.9.1) subfamily. Beta-2/CHRNB2 sub-subfamily. [View classification]

0.1 mL

300 USD