antibody

Rabbit Anti-D4 Receptor Sera

MBS352074

0.1 ml

300 USD

polyclonal

rabbit

nonconjugated

Antibody

Rabbit Anti-D4 Receptor Sera

D-4 Dopamine receptor

D 4 (176-185); D 4 Dopamine Receptor

D(4) dopamine receptor; D(4) dopamine receptor; D(4) dopamine receptor; D(4) dopamine receptor; OTTHUMP00000164435; dopamine D4 receptor; D(2C) dopamine receptor; seven transmembrane helix receptor; dopamine receptor D4; D(2C) dopamine receptor; Dopamine D4 receptor

DRD4; DRD4; D4DR

DRD4_HUMAN

Polyclonal

Rabbit

467

Polypeptide ::: % Cross Reactivity . D4 Dopamine Receptor (Ac-176-185) ::: 100. D4 Dopamine Receptor ::: 60

Antiserum Specificity

Immunocytochemical Staining, Western Immunoblot, Western Blot

Immunocytochemistry: This antiserum has been found to stain specific cells in various regions of PLP fixed rat brain sections at a dilution of 1:10.000. This includes the glomeruli of the olfactory bulb. staining of cortex layer 4 and dendritic staining in cortex layers 2 and 3. In layer 5 of the cortex apical dendrites of many neurons were stained. In the hippocampus diffuse immunoreactivity was observed in the molecular layer of the dentate gyrus. with fine dots associated with many neurons in the granule cell layer. Western Blot: Western immunoblots using whole rat brain homogenate resulted in a single wide band being detected at ~48-53 kD at 1:500 dilution. Western Blot Protocol: 1. After SDS-PAGE on 12% gels and electrophoretic transfer to PVDF membrane. block the membrane overnight with 4% normal goat serum in TBS/Tween-20 buffer. 2. Wash x 2 with TBS/Tween-20. 3. Apply the rabbit polyclonal antibody after dilution to at least 1:500 (Note: higher dilutions may be needed). Use 2% normal goat serum in TBS/Tween-20 as buffer for the primary antibody. Let the primary antibody bind for 2-4 hours. 4. Wash x 3 with TBS/Tween-20. 5. Apply affinity purified HRP-goat anti-rabbit IgG antiserum diluted 1:2500 (dilution may vary depending upon supplier) in 2% normal goat serum in TBS/Tween-20. Incubate 1-2 hours. Note: greater sensitivity may be achieved using ABC techniques. 6. Wash x 4 for 5 minute/wash with TBS/Tween-20. 7. Develop color using the enhanced DAB reaction.

Histochemical Grade Antibodies; Dopamine Receptor Antibodies

The antiserum was raised in a rabbit which was immunized with D4(Ac-176-185) covalently attached onto a carrier protein. and it has been characterized by immunocytochemical. western immunoblot and ELISA techniques. This antiserum has been found to be highly specific for this peptide sequence and is suitable for immunocytochemical detection of the D4 dopamine receptor. Rehydrate the lyophilized antibody with 0.1 ml of PBS which contains 10 mg/ml BSA or with additional buffer (such as 10 mg/ml BSA in PBS) for more dilute antibody

1345939

P21917.2

P21917

P21917

48,361 Da

Amine Ligand-binding Receptors Pathway 106363!!Class A/1 (Rhodopsin-like Receptors) Pathway 106357!!Dopamine Receptors Pathway 106366!!G Alpha (i) Signalling Events Pathway 119550!!GPCR Downstream Signaling Pathway 119548!!GPCR Ligand Binding Pathway 161020!!GPCRs, Class A Rhodopsin-like Pathway 198886!!GPCRs, Other Pathway 198765!!Hypothetical Network For Drug Addiction Pathway 198776!!Monoamine GPCRs Pathway 198861

This gene encodes the D4 subtype of the dopamine receptor. The D4 subtype is a G-protein coupled receptor which inhibits adenylyl cyclase. It is a target for drugs which treat schizophrenia and Parkinson disease. Mutations in this gene have been associated with various behavioral phenotypes, including autonomic nervous system dysfunction, attention deficit/hyperactivity disorder, and the personality trait of novelty seeking. This gene contains a polymorphic number (2-10 copies) of tandem 48 nt repeats; the sequence shown contains four repeats. [provided by RefSeq]

Function: This is one of the five types (D1 to D5) of receptors for dopamine. The activity of this receptor is mediated by G proteins which inhibit adenylyl cyclase. Subunit structure: Interacts with CLIC6. By similarity and GPRASP1. Ref.9. Subcellular location: Cell membrane; Multi-pass membrane protein. Polymorphism: The number of repeats of 16 amino acids in the third cytoplasmic loop is highly polymorphic and varies among different alleles. Alleles corresponding in size to a 2 (D4.2), 3 (D4.3), 4 (D4.4), 5 (D4.5), 6 (D4.6), 7 (D4.7) and 9 (D4.9) repeats have been described. The sequence shown is that of allele D4.7. The polymorphic repeat sequence has little influence on DRD4-binding profiles and might not be essential for G protein interaction. Sequence similarities: Belongs to the G-protein coupled receptor 1 family. Sequence caution: The sequence AAL58637.1 differs from that shown. Reason: Erroneous gene model prediction.

0.1 ml

300 USD