Rabbit Anti-D1 Receptor Sera | MyBioSource MBS352066 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Rabbit Anti-D1 Receptor Sera

catalog :

MBS352066

quantity :

0.1 mL

price :

300 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human

application :

western blot

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS352066

products type :

Antibody

products full name :

Rabbit Anti-D1 Receptor Sera

products short name :

D-1 Dopamine receptor

products name syn :

D1 Receptor (9-21); D1 Dopamine Receptor

other names :

D(1A) dopamine receptor; D(1A) dopamine receptor; D(1A) dopamine receptor; D(1A) dopamine receptor; dopamine D1 receptor; dopamine receptor D1; Dopamine D1 receptor

other gene names :

DRD1; DRD1; DADR; DRD1A

uniprot entry name :

DRD1_HUMAN

clonality :

Polyclonal

host :

Rabbit

sequence length :

446

specificity :

Polypeptide vs % Cross Reactivity. D1 Dopamine Receptor (9-21): 100. D1 Dopamine Receptor: 90. D2 Dopamine Receptor (272-282): 0. D2 Dopamine Receptor: 0. D3 Dopamine Receptor (2-10): 0. D3 Dopamine Receptor: 0. D4 Dopamine Receptor (176-185): 0. D4 Dopamine Receptor: 0. D5 Dopamine Receptor (23-35): 0. D5 Dopamine Receptor: 0

form :

Antiserum Specificity

storage stability :

Store at 2 to 8 degree C.

tested application :

Immunocytochemical Staining, Western Immunoblot, Western Blot

app notes :

Immunocytochemistry: This antiserum has been found to stain specific cells in various regions of PLP fixed rat brain sections at a dilution of 1:5000. This includes the Medial septum. Nucleus accumbens. Dentate gyrus. Globus pallidus. Medial forebrain bundle. Cortex regions 1-3. Substantia Nigra reticulata. and the Ventral tegmental area. Western Blot: Western immunoblots using whole rat brain homogenate resulted in a single band being detected at ~50 kD at 1:1000 dilution. Western Blot Protocol: 1. After SDS-PAGE on 12% gels and electrophoretic transfer to PVDF membrane. block the membrane overnight with 4% normal goat serum in TBS/Tween-20 buffer. 2. Wash x 2 with TBS/Tween-20. 3. Apply the rabbit polyclonal antibody after dilution to at least 1:1000 (Note: higher dilutions may be needed). Use 2% normal goat serum in TBS/Tween-20 as buffer for the primary antibody. Let the primary antibody bind for 2-4 hours. 4. Wash x 3 with TBS/Tween-20. 5. Apply affinity purified HRP-goat anti-rabbit IgG antiserum diluted 1:2500 (dilution may vary depending upon supplier) in 2% normal goat serum in TBS/Tween-20. Incubate 1-2 hours. Note: greater sensitivity may be achieved using ABC techniques. 6. Wash x 4 for 5 minute/wash with TBS/Tween-20. 7. Develop color using the enhanced DAB reaction.

products categories :

Histochemical Grade Antibodies; Dopamine Receptor Antibodies

products description :

The antiserum was raised in a rabbit which was immunized with D1(Ac-9-21-Cys22) covalently attached onto a carrier protein. and it has been characterized by immunocytochemical. western immunoblot and ELISA techniques. The antiserum has been found to be highly specific for this peptide sequence and is suitable for immunocytochemical and western blot detection of the D1 dopamine receptor. Rehydrate the lyophilized antibody with 0.1 ml of PBS which contains 10 mg/ml BSA or with additional buffer (such as 10 mg/ml BSA in PBS) for more dilute antibody

ncbi gi num :

4503383

ncbi acc num :

NP_000785.1

ncbi gb acc num :

NM_000794.3

uniprot acc num :

P21728

ncbi mol weight :

49162 Da

ncbi pathways :

Amine Ligand-binding Receptors Pathway (106363); Calcium Signaling Pathway (83050); Calcium Signaling Pathway (459); Class A/1 (Rhodopsin-like Receptors) Pathway (106357); Dopamine Receptors Pathway (106366); G Alpha (s) Signalling Events Pathway (119549); GPCR Downstream Signaling Pathway (119548); GPCR Ligand Binding Pathway (161020); GPCRs, Class A Rhodopsin-like Pathway (198886); Gap Junction Pathway (83072)

ncbi summary :

This gene encodes the D1 subtype of the dopamine receptor. The D1 subtype is the most abundant dopamine receptor in the central nervous system. This G-protein coupled receptor stimulates adenylyl cyclase and activates cyclic AMP-dependent protein kinases. D1 receptors regulate neuronal growth and development, mediate some behavioral responses, and modulate dopamine receptor D2-mediated events. Alternate transcription initiation sites result in two transcript variants of this gene. [provided by RefSeq]

uniprot summary :

Function: This is one of the five types (D1 to D5) of receptors for dopamine. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase. Subunit structure: Interacts with DNAJC14 via its C-terminus. By similarity. Interacts with DRD1IP. Subcellular location: Cell membrane; Multi-pass membrane protein. By similarity. Endoplasmic reticulum membrane; Multi-pass membrane protein. By similarity. Note: Transport from the endoplasmic reticulum to the cell surface is regulated by interaction with DNAJC14. Tissue specificity: Detected in caudate, nucleus accumbens and in the olfactory tubercle. Ref.1. Sequence similarities: Belongs to the G-protein coupled receptor 1 family.

size1 :

0.1 mL

price1 :

300 USD

more info or order :

MyBioSource product webpage