Anti-SNAP-25 Monoclonal Antibody 5G4-1D1 | MyBioSource MBS350063 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Anti-SNAP-25 Monoclonal Antibody 5G4-1D1

catalog :

MBS350063

quantity :

1 mL

price :

370 USD

clonality :

monoclonal

host :

unidentified

conjugate :

nonconjugated

clone name :

[SNAP-25 (cleaved & uncleaved)]

reactivity :

human

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS350063

products type :

Antibody

products full name :

Anti-SNAP-25 Monoclonal Antibody 5G4-1D1

products short name :

[SNAP-25 (cleaved & uncleaved)]

products name syn :

[SNAP-25 (Cleaved and uncleaved)]

other names :

[Synaptosomal-associated protein 25; Synaptosomal-associated protein 25; synaptosomal-associated protein 25; SUP; super protein; OTTHUMP00000030267; OTTHUMP00000030268; synaptosomal-associated 25 kDa protein; resistance to inhibitors of cholinesterase 4 homolog; synaptosomal-associated protein, 25kDa; Super protein; SUP; Synaptosomal-associated 25 kDa protein]

other gene names :

[SNAP25; SNAP25; RIC4; SEC9; SNAP; RIC-4; SNAP-25; FLJ23079; bA416N4.2; dJ1068F16.2; SNAP]

uniprot entry name :

SNP25_HUMAN

clonality :

Monoclonal

clone :

[SNAP-25 (cleaved & uncleaved)]

host :

unknown

storage stability :

For short term storage, store at 4°C. For long term storage, store frozen at -20°C or colder. Avoid repeated freeze/thaw cycles.

tested application :

Immunocytochemical Staining of Rat Brain Section

app notes :

This monoclonal antibody containing culture supernatant has been found to stain fixed normal rat brain sections in cells known to express SNAP-25 at a dilution of 1:100. The immunocytochemical staining was observed in puncta and at synaptic terminals.

other info1 :

Monoclonal . Antibody. Specifity: Polypeptide: SNAP-25 (uncleaved by BoTox-A). % Cross Reactivity: 100. Polypeptide: SNAP-25 (cleaved by BoTox-A). % Cross Reactivity: 100. Polypeptide: VAMP 2. % Cross Reactivity: 0. Polypeptide: Syntaxin 1. % Cross Reactivity: 0

products categories :

BoTox and BoTox Substrate Antibodies and Kits

products description :

This sterile filtered culture supernatant contains mouse monoclonal antibody 5G4-1D1. a mouse IgG class MAb. that is specific for both the intact whole form of SNAP-25 and the BoTox-A cleaved form of SNAP-25. This monoclonal antibody has been found to bind to cleaved and uncleaved SNAP-25 by ELISA and cell staining techniques. Each vial contains 1.0 ml of culture supernatant

ncbi gi num :

46397726

ncbi acc num :

P60880.1

ncbi gb acc num :

P60880

uniprot acc num :

P60880

ncbi pathways :

Acetylcholine Neurotransmitter Release Cycle Pathway (106525); BoNT Light Chain Types A, C1, E Cleave SNAP-25 Pathway (105738); Botulinum Neurotoxicity Pathway (105734); Diabetes Pathways (105902); Dopamine Neurotransmitter Release Cycle Pathway (106523); Effects Of Botulinum Toxin Pathway (138028); GABA Synthesis, Release, Reuptake And Degradation Pathway (187174); Glutamate Neurotransmitter Release Cycle Pathway (106522); Insulin Signaling Pathway (198845); Insulin Synthesis And Processing Pathway (105903)

ncbi summary :

Synaptic vesicle membrane docking and fusion is mediated by SNAREs (soluble N-ethylmaleimide-sensitive factor attachment protein receptors) located on the vesicle membrane (v-SNAREs) and the target membrane (t-SNAREs). The assembled v-SNARE/t-SNARE complex consists of a bundle of four helices, one of which is supplied by v-SNARE and the other three by t-SNARE. For t-SNAREs on the plasma membrane, the protein syntaxin supplies one helix and the protein encoded by this gene contributes the other two. Therefore, this gene product is a presynaptic plasma membrane protein involved in the regulation of neurotransmitter release. Two alternative transcript variants encoding different protein isoforms have been described for this gene. [provided by RefSeq]

uniprot summary :

Function: t-SNARE involved in the molecular regulation of neurotransmitter release. May play an important role in the synaptic function of specific neuronal systems. Associates with proteins involved in vesicle docking and membrane fusion. Regulates plasma membrane recycling through its interaction with CENPF. Subunit structure: Part of the SNARE core complex containing SNAP25, VAMP2 and STX1A. This complex binds CPLX1. Interacts with CENPF, TRIM9, RIMS1, SNAPIN, OTOF and HGS. Binds STXBP6. Found in a ternary complex with STX1A and VAMP8. Found in a complex containing SYT1, SV2B and syntaxin-1. Subcellular location: Cytoplasm perinuclear region. By similarity. Cell membrane; Lipid-anchor. Cell junction synapse synaptosome. Note: Membrane association requires palmitoylation. Expressed throughout cytoplasm, concentrating at the perinuclear region. Tissue specificity: Neurons of the neocortex, hippocampus, piriform cortex, anterior thalamic nuclei, pontine nuclei, and granule cells of the cerebellum. Post-translational modification: Palmitoylated. Cys-85 appears to be the main site, and palmitoylation is required for membrane association. Miscellaneous: When cloned and expressed in Eschericia coli, where protein palmitoylation does not occur, Cys-85, Cys-88, Cys-90 and Cys-92 in the protein sequence readily form an iron-sulfur cluster instead. Sequence similarities: Belongs to the SNAP-25 family.Contains 2 t-SNARE coiled-coil homology domains.

size1 :

1 mL

price1 :

370 USD

more info or order :

MyBioSource product webpage